Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
The negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of h...
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doaj-e756f8268f1f43ed83aaaf90fdd757b22020-11-25T03:26:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.01037545500Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial PeptideYunfei Wu0Qiushi Liu1Benjamin Weiss2Martin Kaltenpoth3Tatsuhiko Kadowaki4Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, ChinaDepartment of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, ChinaDepartment for Evolutionary Ecology, Institute for Organismic and Molecular Evolution, Johannes Gutenberg University Mainz, Mainz, GermanyDepartment for Evolutionary Ecology, Institute for Organismic and Molecular Evolution, Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, ChinaThe negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of honey bee immune responses stimulated by DWV and the mite to the host (honey bee) and perhaps the pathogen/parasite (DWV/mite) are not yet understood. To answer these questions, we studied the tripartite interactions between the honey bee, Tropilaelaps mercedesae, and DWV as the model. T. mercedesae functioned as a vector for DWV without supporting active viral replication. Thus, DWV negligibly affected mite fitness. Mite infestation induced mRNA expression of antimicrobial peptides (AMPs), Defensin-1 and Hymenoptaecin, which correlated with DWV copy number in honey bee pupae and mite feeding, respectively. Feeding T. mercedesae with fruit fly S2 cells heterologously expressing honey bee Hymenoptaecin significantly downregulated mite Vitellogenin expression, indicating that the honey bee AMP manipulates mite reproduction upon feeding on bee. Our results provide insights into the mechanism of DWV transmission by the honey bee parasitic mite to the host, and the novel role of AMP in defending against mite infestation.https://www.frontiersin.org/article/10.3389/fmicb.2020.01037/fullhost-parasite/pathogen interactionvector-pathogen interactionhoney beeparasitic mitedeformed wing virusantimicrobial peptide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yunfei Wu Qiushi Liu Benjamin Weiss Martin Kaltenpoth Tatsuhiko Kadowaki |
spellingShingle |
Yunfei Wu Qiushi Liu Benjamin Weiss Martin Kaltenpoth Tatsuhiko Kadowaki Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide Frontiers in Microbiology host-parasite/pathogen interaction vector-pathogen interaction honey bee parasitic mite deformed wing virus antimicrobial peptide |
author_facet |
Yunfei Wu Qiushi Liu Benjamin Weiss Martin Kaltenpoth Tatsuhiko Kadowaki |
author_sort |
Yunfei Wu |
title |
Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide |
title_short |
Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide |
title_full |
Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide |
title_fullStr |
Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide |
title_full_unstemmed |
Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide |
title_sort |
honey bee suppresses the parasitic mite vitellogenin by antimicrobial peptide |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-05-01 |
description |
The negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of honey bee immune responses stimulated by DWV and the mite to the host (honey bee) and perhaps the pathogen/parasite (DWV/mite) are not yet understood. To answer these questions, we studied the tripartite interactions between the honey bee, Tropilaelaps mercedesae, and DWV as the model. T. mercedesae functioned as a vector for DWV without supporting active viral replication. Thus, DWV negligibly affected mite fitness. Mite infestation induced mRNA expression of antimicrobial peptides (AMPs), Defensin-1 and Hymenoptaecin, which correlated with DWV copy number in honey bee pupae and mite feeding, respectively. Feeding T. mercedesae with fruit fly S2 cells heterologously expressing honey bee Hymenoptaecin significantly downregulated mite Vitellogenin expression, indicating that the honey bee AMP manipulates mite reproduction upon feeding on bee. Our results provide insights into the mechanism of DWV transmission by the honey bee parasitic mite to the host, and the novel role of AMP in defending against mite infestation. |
topic |
host-parasite/pathogen interaction vector-pathogen interaction honey bee parasitic mite deformed wing virus antimicrobial peptide |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01037/full |
work_keys_str_mv |
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