Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide

The negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of h...

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Main Authors: Yunfei Wu, Qiushi Liu, Benjamin Weiss, Martin Kaltenpoth, Tatsuhiko Kadowaki
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01037/full
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spelling doaj-e756f8268f1f43ed83aaaf90fdd757b22020-11-25T03:26:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.01037545500Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial PeptideYunfei Wu0Qiushi Liu1Benjamin Weiss2Martin Kaltenpoth3Tatsuhiko Kadowaki4Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, ChinaDepartment of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, ChinaDepartment for Evolutionary Ecology, Institute for Organismic and Molecular Evolution, Johannes Gutenberg University Mainz, Mainz, GermanyDepartment for Evolutionary Ecology, Institute for Organismic and Molecular Evolution, Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, ChinaThe negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of honey bee immune responses stimulated by DWV and the mite to the host (honey bee) and perhaps the pathogen/parasite (DWV/mite) are not yet understood. To answer these questions, we studied the tripartite interactions between the honey bee, Tropilaelaps mercedesae, and DWV as the model. T. mercedesae functioned as a vector for DWV without supporting active viral replication. Thus, DWV negligibly affected mite fitness. Mite infestation induced mRNA expression of antimicrobial peptides (AMPs), Defensin-1 and Hymenoptaecin, which correlated with DWV copy number in honey bee pupae and mite feeding, respectively. Feeding T. mercedesae with fruit fly S2 cells heterologously expressing honey bee Hymenoptaecin significantly downregulated mite Vitellogenin expression, indicating that the honey bee AMP manipulates mite reproduction upon feeding on bee. Our results provide insights into the mechanism of DWV transmission by the honey bee parasitic mite to the host, and the novel role of AMP in defending against mite infestation.https://www.frontiersin.org/article/10.3389/fmicb.2020.01037/fullhost-parasite/pathogen interactionvector-pathogen interactionhoney beeparasitic mitedeformed wing virusantimicrobial peptide
collection DOAJ
language English
format Article
sources DOAJ
author Yunfei Wu
Qiushi Liu
Benjamin Weiss
Martin Kaltenpoth
Tatsuhiko Kadowaki
spellingShingle Yunfei Wu
Qiushi Liu
Benjamin Weiss
Martin Kaltenpoth
Tatsuhiko Kadowaki
Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
Frontiers in Microbiology
host-parasite/pathogen interaction
vector-pathogen interaction
honey bee
parasitic mite
deformed wing virus
antimicrobial peptide
author_facet Yunfei Wu
Qiushi Liu
Benjamin Weiss
Martin Kaltenpoth
Tatsuhiko Kadowaki
author_sort Yunfei Wu
title Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
title_short Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
title_full Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
title_fullStr Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
title_full_unstemmed Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide
title_sort honey bee suppresses the parasitic mite vitellogenin by antimicrobial peptide
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2020-05-01
description The negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of honey bee immune responses stimulated by DWV and the mite to the host (honey bee) and perhaps the pathogen/parasite (DWV/mite) are not yet understood. To answer these questions, we studied the tripartite interactions between the honey bee, Tropilaelaps mercedesae, and DWV as the model. T. mercedesae functioned as a vector for DWV without supporting active viral replication. Thus, DWV negligibly affected mite fitness. Mite infestation induced mRNA expression of antimicrobial peptides (AMPs), Defensin-1 and Hymenoptaecin, which correlated with DWV copy number in honey bee pupae and mite feeding, respectively. Feeding T. mercedesae with fruit fly S2 cells heterologously expressing honey bee Hymenoptaecin significantly downregulated mite Vitellogenin expression, indicating that the honey bee AMP manipulates mite reproduction upon feeding on bee. Our results provide insights into the mechanism of DWV transmission by the honey bee parasitic mite to the host, and the novel role of AMP in defending against mite infestation.
topic host-parasite/pathogen interaction
vector-pathogen interaction
honey bee
parasitic mite
deformed wing virus
antimicrobial peptide
url https://www.frontiersin.org/article/10.3389/fmicb.2020.01037/full
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