Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response

Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance prot...

Full description

Bibliographic Details
Main Authors: Xiaoyu Wang, Min Li, Xuming Liu, Lixue Zhang, Qiong Duan, Jixing Zhang
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/20/2/355
id doaj-e763477429264eab81cd0b3e78b3cf68
record_format Article
spelling doaj-e763477429264eab81cd0b3e78b3cf682020-11-25T01:38:53ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-01-0120235510.3390/ijms20020355ijms20020355Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress ResponseXiaoyu Wang0Min Li1Xuming Liu2Lixue Zhang3Qiong Duan4Jixing Zhang5College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaCollege of Agriculture, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaCollege of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaCollege of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaCollege of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaCollege of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaEarly planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C) imbibed seeds. As a result, 127 DAPS were identified according to isobaric tag for relative and absolute quantification (iTRAQ) strategy. These DAPS were mainly involved in carbohydrate and energy metabolism, translation and posttranslational modification, stress response, lipid transport and metabolism, and signal transduction. Enzyme-linked immunosorbent assays (ELISA) demonstrated that the quantitative proteomics data collected here were reliable. This study provided some invaluable insights into the cold stress responses of early imbibed castor seeds: (1) up-accumulation of all DAPS involved in translation might confer cold tolerance by promoting protein synthesis; (2) stress-related proteins probably protect the cell against damage caused by cold stress; (3) up-accumulation of key DAPS associated with fatty acid biosynthesis might facilitate resistance or adaptation of imbibed castor seeds to cold stress by the increased content of unsaturated fatty acid (UFA). The data has been deposited to the ProteomeXchange with identifier PXD010043.http://www.mdpi.com/1422-0067/20/2/355Ricinus communis L.cold stressseed imbibitioniTRAQproteomics
collection DOAJ
language English
format Article
sources DOAJ
author Xiaoyu Wang
Min Li
Xuming Liu
Lixue Zhang
Qiong Duan
Jixing Zhang
spellingShingle Xiaoyu Wang
Min Li
Xuming Liu
Lixue Zhang
Qiong Duan
Jixing Zhang
Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response
International Journal of Molecular Sciences
Ricinus communis L.
cold stress
seed imbibition
iTRAQ
proteomics
author_facet Xiaoyu Wang
Min Li
Xuming Liu
Lixue Zhang
Qiong Duan
Jixing Zhang
author_sort Xiaoyu Wang
title Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response
title_short Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response
title_full Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response
title_fullStr Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response
title_full_unstemmed Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response
title_sort quantitative proteomic analysis of castor (ricinus communis l.) seeds during early imbibition provided novel insights into cold stress response
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-01-01
description Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C) imbibed seeds. As a result, 127 DAPS were identified according to isobaric tag for relative and absolute quantification (iTRAQ) strategy. These DAPS were mainly involved in carbohydrate and energy metabolism, translation and posttranslational modification, stress response, lipid transport and metabolism, and signal transduction. Enzyme-linked immunosorbent assays (ELISA) demonstrated that the quantitative proteomics data collected here were reliable. This study provided some invaluable insights into the cold stress responses of early imbibed castor seeds: (1) up-accumulation of all DAPS involved in translation might confer cold tolerance by promoting protein synthesis; (2) stress-related proteins probably protect the cell against damage caused by cold stress; (3) up-accumulation of key DAPS associated with fatty acid biosynthesis might facilitate resistance or adaptation of imbibed castor seeds to cold stress by the increased content of unsaturated fatty acid (UFA). The data has been deposited to the ProteomeXchange with identifier PXD010043.
topic Ricinus communis L.
cold stress
seed imbibition
iTRAQ
proteomics
url http://www.mdpi.com/1422-0067/20/2/355
work_keys_str_mv AT xiaoyuwang quantitativeproteomicanalysisofcastorricinuscommunislseedsduringearlyimbibitionprovidednovelinsightsintocoldstressresponse
AT minli quantitativeproteomicanalysisofcastorricinuscommunislseedsduringearlyimbibitionprovidednovelinsightsintocoldstressresponse
AT xumingliu quantitativeproteomicanalysisofcastorricinuscommunislseedsduringearlyimbibitionprovidednovelinsightsintocoldstressresponse
AT lixuezhang quantitativeproteomicanalysisofcastorricinuscommunislseedsduringearlyimbibitionprovidednovelinsightsintocoldstressresponse
AT qiongduan quantitativeproteomicanalysisofcastorricinuscommunislseedsduringearlyimbibitionprovidednovelinsightsintocoldstressresponse
AT jixingzhang quantitativeproteomicanalysisofcastorricinuscommunislseedsduringearlyimbibitionprovidednovelinsightsintocoldstressresponse
_version_ 1725051764618559488