SARS-CoV-2-specific immune response in COVID-19 convalescent individuals
Abstract We collected blood from coronavirus disease 2019 (COVID-19) convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients. Follow-up analysis in a cohort of 171 patients at 4–11 months after the onset revealed high levels of IgG ant...
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2021-07-01
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doaj-e76e991d48db4a75a5cfc27796453c862021-07-11T11:11:03ZengNature Publishing GroupSignal Transduction and Targeted Therapy2059-36352021-07-016111010.1038/s41392-021-00686-1SARS-CoV-2-specific immune response in COVID-19 convalescent individualsYunbao Pan0Xianghu Jiang1Liu Yang2Liangjun Chen3Xiaojiao Zeng4Guohong Liu5Yueting Tang6Chungen Qian7Xinming Wang8Fangming Cheng9Jun Lin10Xinghuan Wang11Yirong Li12Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityDepartment of Radiology, Zhongnan Hospital of Wuhan University, Wuhan UniversityDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityThe Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics – Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyReagent R&D Center, Autobio Diagnostics Co., LtdReagent R&D Center, Shenzhen YHLO Biotech Co., LtdWuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical SciencesWuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical SciencesDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan UniversityAbstract We collected blood from coronavirus disease 2019 (COVID-19) convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients. Follow-up analysis in a cohort of 171 patients at 4–11 months after the onset revealed high levels of IgG antibodies. A total of 78.1% (164/210) of the specimens tested positive for neutralizing antibody (NAb). SARS-CoV-2 antigen peptide pools-stimulated-IL-2 and -IFN-γ response can distinguish COVID-19 convalescent individuals from healthy donors. Interestingly, NAb survival was significantly affected by the antigen peptide pools-stimulated-IL-2 response, -IL-8 response, and -IFN-γ response. The antigen peptide pools-activated CD8+ T cell counts were correlated with NAb. The antigen peptide pools-activated natural killer (NK) cell counts in convalescent individuals were correlated with NAb and disease severity. Our data suggested that the development of NAb is associated with the activation of T cells and NK cells. Our work provides a basis for further analysis of the protective immunity to SARS-CoV-2 and for understanding the pathogenesis of COVID-19. It also has implications for the development of an effective vaccine for SARS-CoV-2 infection.https://doi.org/10.1038/s41392-021-00686-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yunbao Pan Xianghu Jiang Liu Yang Liangjun Chen Xiaojiao Zeng Guohong Liu Yueting Tang Chungen Qian Xinming Wang Fangming Cheng Jun Lin Xinghuan Wang Yirong Li |
spellingShingle |
Yunbao Pan Xianghu Jiang Liu Yang Liangjun Chen Xiaojiao Zeng Guohong Liu Yueting Tang Chungen Qian Xinming Wang Fangming Cheng Jun Lin Xinghuan Wang Yirong Li SARS-CoV-2-specific immune response in COVID-19 convalescent individuals Signal Transduction and Targeted Therapy |
author_facet |
Yunbao Pan Xianghu Jiang Liu Yang Liangjun Chen Xiaojiao Zeng Guohong Liu Yueting Tang Chungen Qian Xinming Wang Fangming Cheng Jun Lin Xinghuan Wang Yirong Li |
author_sort |
Yunbao Pan |
title |
SARS-CoV-2-specific immune response in COVID-19 convalescent individuals |
title_short |
SARS-CoV-2-specific immune response in COVID-19 convalescent individuals |
title_full |
SARS-CoV-2-specific immune response in COVID-19 convalescent individuals |
title_fullStr |
SARS-CoV-2-specific immune response in COVID-19 convalescent individuals |
title_full_unstemmed |
SARS-CoV-2-specific immune response in COVID-19 convalescent individuals |
title_sort |
sars-cov-2-specific immune response in covid-19 convalescent individuals |
publisher |
Nature Publishing Group |
series |
Signal Transduction and Targeted Therapy |
issn |
2059-3635 |
publishDate |
2021-07-01 |
description |
Abstract We collected blood from coronavirus disease 2019 (COVID-19) convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients. Follow-up analysis in a cohort of 171 patients at 4–11 months after the onset revealed high levels of IgG antibodies. A total of 78.1% (164/210) of the specimens tested positive for neutralizing antibody (NAb). SARS-CoV-2 antigen peptide pools-stimulated-IL-2 and -IFN-γ response can distinguish COVID-19 convalescent individuals from healthy donors. Interestingly, NAb survival was significantly affected by the antigen peptide pools-stimulated-IL-2 response, -IL-8 response, and -IFN-γ response. The antigen peptide pools-activated CD8+ T cell counts were correlated with NAb. The antigen peptide pools-activated natural killer (NK) cell counts in convalescent individuals were correlated with NAb and disease severity. Our data suggested that the development of NAb is associated with the activation of T cells and NK cells. Our work provides a basis for further analysis of the protective immunity to SARS-CoV-2 and for understanding the pathogenesis of COVID-19. It also has implications for the development of an effective vaccine for SARS-CoV-2 infection. |
url |
https://doi.org/10.1038/s41392-021-00686-1 |
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