MALE ADULT RAT SPERM PARAMETERS AFTER SKELETAL MUSCLE INJURY

• Main Authors: Maicon Borges Moraes, Cassio Noronha Martins, Melina Hauck, Douglas Dalcin Rossato, Carine Dhal Corcini, Antonio Sergio Varela Junior, Luis Ulisses Signori
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Medicina do Esporte
• Series: Revista Brasileira de Medicina do Esporte
• Subjects: Reproducción; Espermatozoides; Músculo esquelético; Inflamación; Estrés oxidativo
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922019000400275&lng=en&tlng=en

ABSTRACT Introduction: Skeletal muscle injuries stimulate a systemic inflammatory response which may interfere in species reproduction. Objective: To evaluate the effects caused by skeletal muscle injuries on the inflammatory response and sperm parameters of male adult rats. Methods: The sample group was composed of 30 Wistar rats distributed evenly across control and injury groups. Muscle injury was induced by bruising, caused by the release of a 200 g weight from a height of 30 cm onto the gastrocnemius muscle. Blood (CBC and damage/muscle inflammation markers), muscle (oxidative stress) and gonad (sperm parameters) samples were collected 72h after the injury. Results: The muscle injury increased monocytes, creatine kinase, C-reactive protein, reactive oxygen species (ROS) concentration and lipid peroxidation. In contrast, the injury reduced antioxidant capacity against peroxyl radicals (ACAP), membrane integrity (36%) and sperm acrosome (33%). Membrane integrity and acrosome (p<0.05) correlate directly with ACAP (ρ=0.602; ρ=0.513 respectively) and inversely with monocytes (ρ=-0.703; ρ=-0.635, respectively), creatine kinase (ρ=-0.450; ρ=-0.603), C-reactive protein (ρ=-0.511; ρ=-0.703) and parameters of oxidative stress (ROS ρ=-0.703; ρ=-0.635; lipid peroxidation ρ=-0.494; ρ=-0.559). Conclusion: The acute systemic inflammatory response arising from skeletal muscle injury interferes in the male reproductive cell organelles (membrane and acrosome). Level of Evidence V; Experimental study.