CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer

CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer

Prostate cancer risk-associated SNPs are enriched in noncoding CREs. Here the authors perform CRISPRi screens of CREs in prostate cancer cell lines to describe a causal mechanism synergistically driven by a risk SNP and DNA methylation-mediated 3D genome architecture.

Bibliographic Details
Main Authors: Musaddeque Ahmed, Fraser Soares, Ji-Han Xia, Yue Yang, Jing Li, Haiyang Guo, Peiran Su, Yijun Tian, Hyung Joo Lee, Miranda Wang, Nayeema Akhtar, Kathleen E. Houlahan, Almudena Bosch, Stanley Zhou, Parisa Mazrooei, Junjie T. Hua, Sujun Chen, Jessica Petricca, Yong Zeng, Alastair Davies, Michael Fraser, David A. Quigley, Felix Y. Feng, Paul C. Boutros, Mathieu Lupien, Amina Zoubeidi, Liang Wang, Martin J. Walsh, Ting Wang, Shancheng Ren, Gong-Hong Wei, Housheng Hansen He
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21867-0
id doaj-e7aa25730939461e8ebe506e7b821266
record_format Article
spelling doaj-e7aa25730939461e8ebe506e7b8212662021-03-21T12:13:20ZengNature Publishing GroupNature Communications2041-17232021-03-0112111510.1038/s41467-021-21867-0CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancerMusaddeque Ahmed0Fraser Soares1Ji-Han Xia2Yue Yang3Jing Li4Haiyang Guo5Peiran Su6Yijun Tian7Hyung Joo Lee8Miranda Wang9Nayeema Akhtar10Kathleen E. Houlahan11Almudena Bosch12Stanley Zhou13Parisa Mazrooei14Junjie T. Hua15Sujun Chen16Jessica Petricca17Yong Zeng18Alastair Davies19Michael Fraser20David A. Quigley21Felix Y. Feng22Paul C. Boutros23Mathieu Lupien24Amina Zoubeidi25Liang Wang26Martin J. Walsh27Ting Wang28Shancheng Ren29Gong-Hong Wei30Housheng Hansen He31Princess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkFaculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of OuluChanghai HospitalChanghai HospitalPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkDepartment of Tumor Biology, H. Lee Moffitt Cancer Center and Research InstituteDepartment of Genetics, Washington University in St. LouisPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkDepartment of Medical Biophysics, University of TorontoDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkPrincess Margaret Cancer Center/University Health NetworkThe Vancouver Prostate Centre, Vancouver General Hospital and Department of Urologic Sciences, The University of British ColumbiaPrincess Margaret Cancer Center/University Health NetworkHelen Diller Family Comprehensive Cancer Center, University of California at San FranciscoHelen Diller Family Comprehensive Cancer Center, University of California at San FranciscoVector InstitutePrincess Margaret Cancer Center/University Health NetworkThe Vancouver Prostate Centre, Vancouver General Hospital and Department of Urologic Sciences, The University of British ColumbiaDepartment of Tumor Biology, H. Lee Moffitt Cancer Center and Research InstituteDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Genetics, Washington University in St. LouisChanghai HospitalFaculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of OuluPrincess Margaret Cancer Center/University Health NetworkProstate cancer risk-associated SNPs are enriched in noncoding CREs. Here the authors perform CRISPRi screens of CREs in prostate cancer cell lines to describe a causal mechanism synergistically driven by a risk SNP and DNA methylation-mediated 3D genome architecture.https://doi.org/10.1038/s41467-021-21867-0
collection DOAJ
language English
format Article
sources DOAJ
author Musaddeque Ahmed
Fraser Soares
Ji-Han Xia
Yue Yang
Jing Li
Haiyang Guo
Peiran Su
Yijun Tian
Hyung Joo Lee
Miranda Wang
Nayeema Akhtar
Kathleen E. Houlahan
Almudena Bosch
Stanley Zhou
Parisa Mazrooei
Junjie T. Hua
Sujun Chen
Jessica Petricca
Yong Zeng
Alastair Davies
Michael Fraser
David A. Quigley
Felix Y. Feng
Paul C. Boutros
Mathieu Lupien
Amina Zoubeidi
Liang Wang
Martin J. Walsh
Ting Wang
Shancheng Ren
Gong-Hong Wei
Housheng Hansen He
spellingShingle Musaddeque Ahmed
Fraser Soares
Ji-Han Xia
Yue Yang
Jing Li
Haiyang Guo
Peiran Su
Yijun Tian
Hyung Joo Lee
Miranda Wang
Nayeema Akhtar
Kathleen E. Houlahan
Almudena Bosch
Stanley Zhou
Parisa Mazrooei
Junjie T. Hua
Sujun Chen
Jessica Petricca
Yong Zeng
Alastair Davies
Michael Fraser
David A. Quigley
Felix Y. Feng
Paul C. Boutros
Mathieu Lupien
Amina Zoubeidi
Liang Wang
Martin J. Walsh
Ting Wang
Shancheng Ren
Gong-Hong Wei
Housheng Hansen He
CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
Nature Communications
author_facet Musaddeque Ahmed
Fraser Soares
Ji-Han Xia
Yue Yang
Jing Li
Haiyang Guo
Peiran Su
Yijun Tian
Hyung Joo Lee
Miranda Wang
Nayeema Akhtar
Kathleen E. Houlahan
Almudena Bosch
Stanley Zhou
Parisa Mazrooei
Junjie T. Hua
Sujun Chen
Jessica Petricca
Yong Zeng
Alastair Davies
Michael Fraser
David A. Quigley
Felix Y. Feng
Paul C. Boutros
Mathieu Lupien
Amina Zoubeidi
Liang Wang
Martin J. Walsh
Ting Wang
Shancheng Ren
Gong-Hong Wei
Housheng Hansen He
author_sort Musaddeque Ahmed
title CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
title_short CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
title_full CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
title_fullStr CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
title_full_unstemmed CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
title_sort crispri screens reveal a dna methylation-mediated 3d genome dependent causal mechanism in prostate cancer
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-03-01
description Prostate cancer risk-associated SNPs are enriched in noncoding CREs. Here the authors perform CRISPRi screens of CREs in prostate cancer cell lines to describe a causal mechanism synergistically driven by a risk SNP and DNA methylation-mediated 3D genome architecture.
url https://doi.org/10.1038/s41467-021-21867-0
work_keys_str_mv AT musaddequeahmed crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT frasersoares crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT jihanxia crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT yueyang crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT jingli crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT haiyangguo crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT peiransu crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT yijuntian crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT hyungjoolee crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT mirandawang crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT nayeemaakhtar crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT kathleenehoulahan crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT almudenabosch crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT stanleyzhou crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT parisamazrooei crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT junjiethua crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT sujunchen crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT jessicapetricca crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT yongzeng crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT alastairdavies crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT michaelfraser crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT davidaquigley crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT felixyfeng crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT paulcboutros crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT mathieulupien crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT aminazoubeidi crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT liangwang crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT martinjwalsh crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT tingwang crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT shanchengren crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT gonghongwei crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
AT houshenghansenhe crispriscreensrevealadnamethylationmediated3dgenomedependentcausalmechanisminprostatecancer
_version_ 1724210804997226496

Similar Items