Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
The cortical polarity regulators PAR-6, PKC-3, and PAR-3 are essential for the polarization of a broad variety of cell types in multicellular animals. In C. elegans, the roles of the PAR proteins in embryonic development have been extensively studied, yet little is known about their functions during...
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doaj-e7c0a141a08144dc99ceee6465988acf2021-05-05T21:50:50ZengeLife Sciences Publications LtdeLife2050-084X2020-12-01910.7554/eLife.62067Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubulesVictoria G Castiglioni0https://orcid.org/0000-0001-9884-2537Helena R Pires1https://orcid.org/0000-0001-8351-0812Rodrigo Rosas Bertolini2Amalia Riga3Jana Kerver4Mike Boxem5https://orcid.org/0000-0003-3966-4173Division of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NetherlandsThe cortical polarity regulators PAR-6, PKC-3, and PAR-3 are essential for the polarization of a broad variety of cell types in multicellular animals. In C. elegans, the roles of the PAR proteins in embryonic development have been extensively studied, yet little is known about their functions during larval development. Using inducible protein degradation, we show that PAR-6 and PKC-3, but not PAR-3, are essential for postembryonic development. PAR-6 and PKC-3 are required in the epidermal epithelium for animal growth, molting, and the proper pattern of seam-cell divisions. Finally, we uncovered a novel role for PAR-6 in organizing non-centrosomal microtubule arrays in the epidermis. PAR-6 was required for the localization of the microtubule organizer NOCA-1/Ninein, and defects in a noca-1 mutant are highly similar to those caused by epidermal PAR-6 depletion. As NOCA-1 physically interacts with PAR-6, we propose that PAR-6 promotes non-centrosomal microtubule organization through localization of NOCA-1/Ninein.https://elifesciences.org/articles/62067cell polarityPar3Par6aPKCmicrotubuleNOCA-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Victoria G Castiglioni Helena R Pires Rodrigo Rosas Bertolini Amalia Riga Jana Kerver Mike Boxem |
spellingShingle |
Victoria G Castiglioni Helena R Pires Rodrigo Rosas Bertolini Amalia Riga Jana Kerver Mike Boxem Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules eLife cell polarity Par3 Par6 aPKC microtubule NOCA-1 |
author_facet |
Victoria G Castiglioni Helena R Pires Rodrigo Rosas Bertolini Amalia Riga Jana Kerver Mike Boxem |
author_sort |
Victoria G Castiglioni |
title |
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules |
title_short |
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules |
title_full |
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules |
title_fullStr |
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules |
title_full_unstemmed |
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules |
title_sort |
epidermal par-6 and pkc-3 are essential for larval development of c. elegans and organize non-centrosomal microtubules |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-12-01 |
description |
The cortical polarity regulators PAR-6, PKC-3, and PAR-3 are essential for the polarization of a broad variety of cell types in multicellular animals. In C. elegans, the roles of the PAR proteins in embryonic development have been extensively studied, yet little is known about their functions during larval development. Using inducible protein degradation, we show that PAR-6 and PKC-3, but not PAR-3, are essential for postembryonic development. PAR-6 and PKC-3 are required in the epidermal epithelium for animal growth, molting, and the proper pattern of seam-cell divisions. Finally, we uncovered a novel role for PAR-6 in organizing non-centrosomal microtubule arrays in the epidermis. PAR-6 was required for the localization of the microtubule organizer NOCA-1/Ninein, and defects in a noca-1 mutant are highly similar to those caused by epidermal PAR-6 depletion. As NOCA-1 physically interacts with PAR-6, we propose that PAR-6 promotes non-centrosomal microtubule organization through localization of NOCA-1/Ninein. |
topic |
cell polarity Par3 Par6 aPKC microtubule NOCA-1 |
url |
https://elifesciences.org/articles/62067 |
work_keys_str_mv |
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