Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers t...

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Main Authors:	Vincent D. Ching-Roa, Eben M. Olson, Sherrif F. Ibrahim, Richard Torres, Michael G. Giacomelli
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-03-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-021-84522-0

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spelling	doaj-e7deef1b6b114f769527cbff79fb3da82021-03-11T12:24:39ZengNature Publishing GroupScientific Reports2045-23222021-03-0111111210.1038/s41598-021-84522-0Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliersVincent D. Ching-Roa0Eben M. Olson1Sherrif F. Ibrahim2Richard Torres3Michael G. Giacomelli4Department of Biomedical Engineering, University of RochesterDepartment of Laboratory Medicine, Yale UniversityDepartment of Dermatology, University of Rochester Medical CenterDepartment of Laboratory Medicine, Yale UniversityDepartment of Biomedical Engineering, University of RochesterAbstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy.https://doi.org/10.1038/s41598-021-84522-0
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli
spellingShingle	Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers Scientific Reports
author_facet	Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli
author_sort	Vincent D. Ching-Roa
title	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_short	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_full	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_fullStr	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_full_unstemmed	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_sort	ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2021-03-01
description	Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy.
url	https://doi.org/10.1038/s41598-021-84522-0
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Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

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