Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers t...
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2021-03-01
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Online Access: | https://doi.org/10.1038/s41598-021-84522-0 |
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doaj-e7deef1b6b114f769527cbff79fb3da82021-03-11T12:24:39ZengNature Publishing GroupScientific Reports2045-23222021-03-0111111210.1038/s41598-021-84522-0Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliersVincent D. Ching-Roa0Eben M. Olson1Sherrif F. Ibrahim2Richard Torres3Michael G. Giacomelli4Department of Biomedical Engineering, University of RochesterDepartment of Laboratory Medicine, Yale UniversityDepartment of Dermatology, University of Rochester Medical CenterDepartment of Laboratory Medicine, Yale UniversityDepartment of Biomedical Engineering, University of RochesterAbstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy.https://doi.org/10.1038/s41598-021-84522-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli |
spellingShingle |
Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers Scientific Reports |
author_facet |
Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli |
author_sort |
Vincent D. Ching-Roa |
title |
Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers |
title_short |
Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers |
title_full |
Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers |
title_fullStr |
Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers |
title_full_unstemmed |
Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers |
title_sort |
ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-03-01 |
description |
Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy. |
url |
https://doi.org/10.1038/s41598-021-84522-0 |
work_keys_str_mv |
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