Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study
The search of suitable combinations of stem cells, biomaterials and scaffolds manufacturing methods have become a major focus of research for bone engineering. The aim of this study was to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed...
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doaj-e7f2e779f63446fcb51170d635d8df7b2021-04-04T23:01:03ZengMDPI AGPolymers2073-43602021-04-01131154115410.3390/polym13071154Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro StudyRaúl Rosales-Ibáñez0Nieves Cubo-Mateo1Amairany Rodríguez-Navarrete2Arely M. González-González3Tomás E. Villamar-Duque4Leticia O. Flores-Sánchez5Luis M. Rodríguez-Lorenzo6Tissue Engineering Lab, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, Ciudad de México CP. 07239, MexicoSensors and Ultrasonic Systems Department, Institute for Physical and Information Technologies, (ITEFI-CSIC), C/ Serrano 144, 28006 Madrid, SpainTissue Engineering Lab, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, Ciudad de México CP. 07239, MexicoTissue Engineering Lab, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, Ciudad de México CP. 07239, MexicoBioterio, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. De los Barrios No.1, Tlalnepantla, Estado de México CP. 54090, MexicoBioterio, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. De los Barrios No.1, Tlalnepantla, Estado de México CP. 54090, MexicoDepartment of Polymeric Nanomaterials and Biomaterials, Institute Science and Technology of Polymers (ICTP-CSIC), C/ Juan de la Cierva 3, 28006 Madrid, SpainThe search of suitable combinations of stem cells, biomaterials and scaffolds manufacturing methods have become a major focus of research for bone engineering. The aim of this study was to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. A 100% pure M<sub>w</sub>: 84,500 ± 1000 PCL was selected. 5 × 10 × 5 mm<sup>3</sup> parallelepiped scaffolds were designed as a wood-pilled structure composed of 20 layers of 250 μm in height, in a non-alternate order ([0,0,0,90,90,90°]). 3D printing was made at 170 °C. Swine dental pulp stem cells (DPSCs) were extracted from lower lateral incisors of swine and cultivated until the cells reached 80% confluence. The third passage was used for seeding on the scaffolds. Phenotype of cells was determined by flow Cytometry<b>.</b> Live and dead, Alamar blue™, von Kossa and alizarin red staining assays were performed. Scaffolds with 290 + 30 μm strand diameter, 938 ± 80 μm pores in the axial direction and 689 ± 13 μm pores in the lateral direction were manufactured. Together, cell viability tests, von Kossa and Alizarin red staining indicate the ability of the printed scaffolds to support DPSCs attachment, proliferation and enable differentiation followed by mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be though to be used for further modelling and preclinical experiments in bone engineering studies.https://www.mdpi.com/2073-4360/13/7/1154polycaprolactonedental pulp stem cells (DPSCs)3D printingscaffoldsbone regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raúl Rosales-Ibáñez Nieves Cubo-Mateo Amairany Rodríguez-Navarrete Arely M. González-González Tomás E. Villamar-Duque Leticia O. Flores-Sánchez Luis M. Rodríguez-Lorenzo |
spellingShingle |
Raúl Rosales-Ibáñez Nieves Cubo-Mateo Amairany Rodríguez-Navarrete Arely M. González-González Tomás E. Villamar-Duque Leticia O. Flores-Sánchez Luis M. Rodríguez-Lorenzo Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study Polymers polycaprolactone dental pulp stem cells (DPSCs) 3D printing scaffolds bone regeneration |
author_facet |
Raúl Rosales-Ibáñez Nieves Cubo-Mateo Amairany Rodríguez-Navarrete Arely M. González-González Tomás E. Villamar-Duque Leticia O. Flores-Sánchez Luis M. Rodríguez-Lorenzo |
author_sort |
Raúl Rosales-Ibáñez |
title |
Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study |
title_short |
Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study |
title_full |
Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study |
title_fullStr |
Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study |
title_full_unstemmed |
Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study |
title_sort |
assessment of a pcl-3d printing-dental pulp stem cells triplet for bone engineering: an in vitro study |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2021-04-01 |
description |
The search of suitable combinations of stem cells, biomaterials and scaffolds manufacturing methods have become a major focus of research for bone engineering. The aim of this study was to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. A 100% pure M<sub>w</sub>: 84,500 ± 1000 PCL was selected. 5 × 10 × 5 mm<sup>3</sup> parallelepiped scaffolds were designed as a wood-pilled structure composed of 20 layers of 250 μm in height, in a non-alternate order ([0,0,0,90,90,90°]). 3D printing was made at 170 °C. Swine dental pulp stem cells (DPSCs) were extracted from lower lateral incisors of swine and cultivated until the cells reached 80% confluence. The third passage was used for seeding on the scaffolds. Phenotype of cells was determined by flow Cytometry<b>.</b> Live and dead, Alamar blue™, von Kossa and alizarin red staining assays were performed. Scaffolds with 290 + 30 μm strand diameter, 938 ± 80 μm pores in the axial direction and 689 ± 13 μm pores in the lateral direction were manufactured. Together, cell viability tests, von Kossa and Alizarin red staining indicate the ability of the printed scaffolds to support DPSCs attachment, proliferation and enable differentiation followed by mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be though to be used for further modelling and preclinical experiments in bone engineering studies. |
topic |
polycaprolactone dental pulp stem cells (DPSCs) 3D printing scaffolds bone regeneration |
url |
https://www.mdpi.com/2073-4360/13/7/1154 |
work_keys_str_mv |
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