Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China
Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen conven...
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doaj-e7fe1563b80846fa96a4584f83ad974a2021-07-02T02:03:01ZengHindawi LimitedCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932016-01-01201610.1155/2016/91838619183861Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of ChinaHaixiang Qiu0Patrick John Kelly1Jilei Zhang2Qinghua Luo3Yi Yang4Yongjiang Mao5Zhangping Yang6Jing Li7Hongzhuan Wu8Chengming Wang9Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaRoss University School of Veterinary Medicine, Basseterre 00265, Saint Kitts and NevisJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaYangzhou University College of Animal Science and Technology, Yangzhou, Jiangsu 225009, ChinaYangzhou University College of Animal Science and Technology, Yangzhou, Jiangsu 225009, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaAlabama State University, Montgomery, AL 36104, USADepartment of Pathobiology, Auburn University College of Veterinary Medicine, Auburn, AL 36849, USAAnaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma spp. and Ehrlichia spp. Positive samples were further analyzed with a standard PCR for the gltA. Anaplasma spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). Ehrlichia spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of gltA PCR products showed that A. marginale, A. ovis, Ehrlichia canis, and Ehrlichia sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. BL126-13 (KJ410243), and Anaplasma sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of Anaplasma spp. and Ehrlichia spp.http://dx.doi.org/10.1155/2016/9183861 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haixiang Qiu Patrick John Kelly Jilei Zhang Qinghua Luo Yi Yang Yongjiang Mao Zhangping Yang Jing Li Hongzhuan Wu Chengming Wang |
spellingShingle |
Haixiang Qiu Patrick John Kelly Jilei Zhang Qinghua Luo Yi Yang Yongjiang Mao Zhangping Yang Jing Li Hongzhuan Wu Chengming Wang Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China Canadian Journal of Infectious Diseases and Medical Microbiology |
author_facet |
Haixiang Qiu Patrick John Kelly Jilei Zhang Qinghua Luo Yi Yang Yongjiang Mao Zhangping Yang Jing Li Hongzhuan Wu Chengming Wang |
author_sort |
Haixiang Qiu |
title |
Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China |
title_short |
Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China |
title_full |
Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China |
title_fullStr |
Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China |
title_full_unstemmed |
Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China |
title_sort |
molecular detection of anaplasma spp. and ehrlichia spp. in ruminants from twelve provinces of china |
publisher |
Hindawi Limited |
series |
Canadian Journal of Infectious Diseases and Medical Microbiology |
issn |
1712-9532 1918-1493 |
publishDate |
2016-01-01 |
description |
Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma spp. and Ehrlichia spp. Positive samples were further analyzed with a standard PCR for the gltA. Anaplasma spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). Ehrlichia spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of gltA PCR products showed that A. marginale, A. ovis, Ehrlichia canis, and Ehrlichia sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. BL126-13 (KJ410243), and Anaplasma sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of Anaplasma spp. and Ehrlichia spp. |
url |
http://dx.doi.org/10.1155/2016/9183861 |
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