Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis
This review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell....
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doaj-e7ff59b27bb3472b9f990957218b749e2020-11-24T21:16:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-07-01168167631677710.3390/ijms160816763ijms160816763Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell AnalysisShih-Jie Lo0Da-Jeng Yao1Institute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 300, TaiwanInstitute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 300, TaiwanThis review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell. This review aims to introduce microfluidic related techniques for the isolation, trapping and manipulation of a single cell. The major approaches for detection in single-cell analysis are introduced; the applications of single-cell analysis are then summarized. The review concludes with discussions of the future directions and opportunities of microfluidic systems applied in analysis of a single cell.http://www.mdpi.com/1422-0067/16/8/16763microfluidic techniquessingle-cell manipulationsingle-cell analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shih-Jie Lo Da-Jeng Yao |
spellingShingle |
Shih-Jie Lo Da-Jeng Yao Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis International Journal of Molecular Sciences microfluidic techniques single-cell manipulation single-cell analysis |
author_facet |
Shih-Jie Lo Da-Jeng Yao |
author_sort |
Shih-Jie Lo |
title |
Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis |
title_short |
Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis |
title_full |
Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis |
title_fullStr |
Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis |
title_full_unstemmed |
Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis |
title_sort |
get to understand more from single-cells: current studies of microfluidic-based techniques for single-cell analysis |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-07-01 |
description |
This review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell. This review aims to introduce microfluidic related techniques for the isolation, trapping and manipulation of a single cell. The major approaches for detection in single-cell analysis are introduced; the applications of single-cell analysis are then summarized. The review concludes with discussions of the future directions and opportunities of microfluidic systems applied in analysis of a single cell. |
topic |
microfluidic techniques single-cell manipulation single-cell analysis |
url |
http://www.mdpi.com/1422-0067/16/8/16763 |
work_keys_str_mv |
AT shihjielo gettounderstandmorefromsinglecellscurrentstudiesofmicrofluidicbasedtechniquesforsinglecellanalysis AT dajengyao gettounderstandmorefromsinglecellscurrentstudiesofmicrofluidicbasedtechniquesforsinglecellanalysis |
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1726017079920820224 |