Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation
PIWI-LIKE 2, a member of the ARGONAUTE protein family, is exclusively expressed in pre-pachytene and pachytene stages of spermatogenesis. PIWI-LIKE 2 acts in the germ cell development and the silencing of retrotransponsons to maintain the genomic integrity and stem cell character. In the present stu...
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doaj-e81ba9195f9c4270a5903e9cf12a18832020-11-25T00:45:21ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-09-01910.3389/fgene.2018.00375388393Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter MethylationMaria GieblerThomas GreitherHermann M. BehrePIWI-LIKE 2, a member of the ARGONAUTE protein family, is exclusively expressed in pre-pachytene and pachytene stages of spermatogenesis. PIWI-LIKE 2 acts in the germ cell development and the silencing of retrotransponsons to maintain the genomic integrity and stem cell character. In the present study we investigated DNA methylation as potential mechanism for the regulation of human PIWI-LIKE 2 expression in cell lines related to spermatozoa precursor cells. We detected a high methylation of the PIWI-LIKE 2 promoter in TCam-2 cells, while in NT2/D1 cells the promoter was hypomethylated. Concordantly, PIWI-LIKE 2 expression is higher in NT2/D1 cells than in TCam-2 cells. By demethylation of the promoter with 5′-Aza-2′-deoxycytidine, PIWI-LIKE 2 expression in TCam-2 was increased, while in NT2/D1 no alterations in PIWI-LIKE 2 expression could be detected. In conclusion, we analyzed the DNA methylation driving PIWI-LIKE 2 expression in undifferentiated germ cell tumors and demonstrated an epigenetic basis for PIWI-LIKE 2 expression in this cell type.https://www.frontiersin.org/article/10.3389/fgene.2018.00375/fullepigeneticsPIWI-LIKE 2germ cell tumorspromoter methylationspermatogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Giebler Thomas Greither Hermann M. Behre |
spellingShingle |
Maria Giebler Thomas Greither Hermann M. Behre Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation Frontiers in Genetics epigenetics PIWI-LIKE 2 germ cell tumors promoter methylation spermatogenesis |
author_facet |
Maria Giebler Thomas Greither Hermann M. Behre |
author_sort |
Maria Giebler |
title |
Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation |
title_short |
Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation |
title_full |
Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation |
title_fullStr |
Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation |
title_full_unstemmed |
Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation |
title_sort |
differential regulation of piwi-like 2 expression in primordial germ cell tumor cell lines by promoter methylation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2018-09-01 |
description |
PIWI-LIKE 2, a member of the ARGONAUTE protein family, is exclusively expressed in pre-pachytene and pachytene stages of spermatogenesis. PIWI-LIKE 2 acts in the germ cell development and the silencing of retrotransponsons to maintain the genomic integrity and stem cell character. In the present study we investigated DNA methylation as potential mechanism for the regulation of human PIWI-LIKE 2 expression in cell lines related to spermatozoa precursor cells. We detected a high methylation of the PIWI-LIKE 2 promoter in TCam-2 cells, while in NT2/D1 cells the promoter was hypomethylated. Concordantly, PIWI-LIKE 2 expression is higher in NT2/D1 cells than in TCam-2 cells. By demethylation of the promoter with 5′-Aza-2′-deoxycytidine, PIWI-LIKE 2 expression in TCam-2 was increased, while in NT2/D1 no alterations in PIWI-LIKE 2 expression could be detected. In conclusion, we analyzed the DNA methylation driving PIWI-LIKE 2 expression in undifferentiated germ cell tumors and demonstrated an epigenetic basis for PIWI-LIKE 2 expression in this cell type. |
topic |
epigenetics PIWI-LIKE 2 germ cell tumors promoter methylation spermatogenesis |
url |
https://www.frontiersin.org/article/10.3389/fgene.2018.00375/full |
work_keys_str_mv |
AT mariagiebler differentialregulationofpiwilike2expressioninprimordialgermcelltumorcelllinesbypromotermethylation AT thomasgreither differentialregulationofpiwilike2expressioninprimordialgermcelltumorcelllinesbypromotermethylation AT hermannmbehre differentialregulationofpiwilike2expressioninprimordialgermcelltumorcelllinesbypromotermethylation |
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1725270607123185664 |