Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages

• Main Authors: Péter Fűtő, Nóra Adányi, Attila Kiss, Gábor Csiffáry
• Format: Article
• Language: English
• Published: University of Zagreb 2016-01-01
• Series: Food Technology and Biotechnology
• Subjects: biosensor; l-ascorbic acid; ascorbate oxidase; screen-printed carbon electrodes
• Online Access: http://hrcak.srce.hr/file/228597

A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 μmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements.