Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing
Metal corrosion is of worldwide concern because it is the cause of major economic losses, and because it creates significant safety issues. The mechanism of the corrosion process, as influenced by bacteria, has been studied extensively. However, the bacterial communities that create the biofilms tha...
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doaj-e82695e0d40b483ca9f3187b326e9e032020-11-24T21:10:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01737282290Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq SequencingXiaohong Li0Xiaohong Li1Jizhou Duan2Hui Xiao3Yongqian Li4Yongqian Li5Haixia Liu6Fang Guan7Xiaofan Zhai8Key Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of SciencesQingdao, ChinaCollege of Marine Life Sciences, Ocean University of ChinaQingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of SciencesQingdao, ChinaCollege of Marine Life Sciences, Ocean University of ChinaQingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of SciencesQingdao, ChinaCollege of Marine Life Sciences, Ocean University of ChinaQingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of SciencesQingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of SciencesQingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of SciencesQingdao, ChinaMetal corrosion is of worldwide concern because it is the cause of major economic losses, and because it creates significant safety issues. The mechanism of the corrosion process, as influenced by bacteria, has been studied extensively. However, the bacterial communities that create the biofilms that form on metals are complicated, and have not been well studied. This is why we sought to analyze the composition of bacterial communities living on steel structures, together with the influence of ecological factors on these communities. The corrosion samples were collected from rust layers on steel plates that were immersed in seawater for two different periods at Sanya and Xiamen, China. We analyzed the bacterial communities on the samples by targeted 16S rRNA gene (V3–V4 region) sequencing using the Illumina MiSeq. Phylogenetic analysis revealed that the bacteria fell into 13 phylotypes (similarity level = 97%). Proteobacteria, Firmicutes and Bacteroidetes were the dominant phyla, accounting for 88.84% of the total. Deltaproteobacteria, Clostridia and Gammaproteobacteria were the dominant classes, and accounted for 70.90% of the total. Desulfovibrio spp., Desulfobacter spp. and Desulfotomaculum spp. were the dominant genera and accounted for 45.87% of the total. These genera are sulfate-reducing bacteria that are known to corrode steel. Bacterial diversity on the 6 months immersion samples was much higher than that of the samples that had been immersed for 8 years (P < 0.001, Student’s t-test). The average complexity of the biofilms from the 8-years immersion samples from Sanya was greater than those from Xiamen, but not significantly so (P > 0.05, Student’s t-test). Overall, the data showed that the rust layers on the steel plates carried many bacterial species. The bacterial community composition was influenced by the immersion time. The results of our study will be of benefit to the further studies of bacterial corrosion mechanisms and corrosion resistance.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01737/fullbacterial communityMICcarbon steelIllumina MiSeq sequencing16S rRNA gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaohong Li Xiaohong Li Jizhou Duan Hui Xiao Yongqian Li Yongqian Li Haixia Liu Fang Guan Xiaofan Zhai |
spellingShingle |
Xiaohong Li Xiaohong Li Jizhou Duan Hui Xiao Yongqian Li Yongqian Li Haixia Liu Fang Guan Xiaofan Zhai Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing Frontiers in Microbiology bacterial community MIC carbon steel Illumina MiSeq sequencing 16S rRNA gene |
author_facet |
Xiaohong Li Xiaohong Li Jizhou Duan Hui Xiao Yongqian Li Yongqian Li Haixia Liu Fang Guan Xiaofan Zhai |
author_sort |
Xiaohong Li |
title |
Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing |
title_short |
Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing |
title_full |
Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing |
title_fullStr |
Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing |
title_full_unstemmed |
Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing |
title_sort |
analysis of bacterial community composition of corroded steel immersed in sanya and xiamen seawaters in china via method of illumina miseq sequencing |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-09-01 |
description |
Metal corrosion is of worldwide concern because it is the cause of major economic losses, and because it creates significant safety issues. The mechanism of the corrosion process, as influenced by bacteria, has been studied extensively. However, the bacterial communities that create the biofilms that form on metals are complicated, and have not been well studied. This is why we sought to analyze the composition of bacterial communities living on steel structures, together with the influence of ecological factors on these communities. The corrosion samples were collected from rust layers on steel plates that were immersed in seawater for two different periods at Sanya and Xiamen, China. We analyzed the bacterial communities on the samples by targeted 16S rRNA gene (V3–V4 region) sequencing using the Illumina MiSeq. Phylogenetic analysis revealed that the bacteria fell into 13 phylotypes (similarity level = 97%). Proteobacteria, Firmicutes and Bacteroidetes were the dominant phyla, accounting for 88.84% of the total. Deltaproteobacteria, Clostridia and Gammaproteobacteria were the dominant classes, and accounted for 70.90% of the total. Desulfovibrio spp., Desulfobacter spp. and Desulfotomaculum spp. were the dominant genera and accounted for 45.87% of the total. These genera are sulfate-reducing bacteria that are known to corrode steel. Bacterial diversity on the 6 months immersion samples was much higher than that of the samples that had been immersed for 8 years (P < 0.001, Student’s t-test). The average complexity of the biofilms from the 8-years immersion samples from Sanya was greater than those from Xiamen, but not significantly so (P > 0.05, Student’s t-test). Overall, the data showed that the rust layers on the steel plates carried many bacterial species. The bacterial community composition was influenced by the immersion time. The results of our study will be of benefit to the further studies of bacterial corrosion mechanisms and corrosion resistance. |
topic |
bacterial community MIC carbon steel Illumina MiSeq sequencing 16S rRNA gene |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01737/full |
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