Investigation the Effects of Lactobacillus acidophilus and Lacto-bacillus casei on aflR Gene expression in Aspergillus parasiticus by Real Time-PCR
<p><strong>Background:</strong> The effect of probiotic bacteria (<em>Lactobacillus acidophilus</em> and <em>L. casei</em>)<strong> </strong>as safe organisms was examined on fungal growth and aflatoxin gene regulation in <em>Aspergillus pa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2016-08-01
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| Series: | Iranian Journal of Public Health |
| Online Access: | http://ijph.tums.ac.ir/index.php/ijph/article/view/7084 |
| Summary: | <p><strong>Background:</strong> The effect of probiotic bacteria (<em>Lactobacillus acidophilus</em> and <em>L. casei</em>)<strong> </strong>as safe organisms was examined on fungal growth and aflatoxin gene regulation in <em>Aspergillus parasiticus</em>.</p><p><strong>Methods</strong><strong>:</strong> The fungus was cultured in presence of two different concentrations of <em>L. acidophilus</em> and <em>L. casei</em> in MRS broth medium. Mycelia dry weight is indicated as criteria to evaluate fungal growth. Besides, investigation of aflR gene expression by Real Time PCR was performed for analysis of gene regulatory effects in aflatoxin biosynthetic pathway.</p><p><strong>Results</strong><strong>:</strong> Both <em>Lactobacillus</em> strongly inhibited fungal growth in the concentrations of 1. 5 10<sup>2</sup>, 1. 5 10<sup>3</sup> . Expression analysis of aflatoxin genes pathway by real time PCR showed inhibitory effect of <em>L. acidophilus</em> and <em>L. casei</em> on expression of aflR gene. The gene expression revealed to be reduced at the approximate rates of 99. 7% and 98% respectively by <em>L. acidopholus</em> and <em>L. casei</em> in concentrations of 1. 5 10<sup>2</sup> and more.</p><p><strong>Conclusion</strong><strong>:</strong> <em>L. acidophilus</em> and <em>L. casei</em> may be used successfully as suitable candidates in controlling of <em>A. parasiticus</em> growth on food and feed as well as reducing of aflatoxin contamination.</p><strong>Keywords:</strong> Aflatoxin, <em>Aspergillus parasiticus</em>, <em>Lactobacilus acidophilus</em>, <em>Lactobacilus casei</em>, |
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| ISSN: | 2251-6085 2251-6093 |