Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar
Shoot branching is an important adaptive trait that determines plant architecture. In a previous study, the Early bud-break 1 (EBB1) gene in peach (Prunus persica var. nectarina) cultivar Zhongyou 4 was transformed into poplar (Populus trichocarpa). PpEBB1-oe poplar showed a more branched phenotype....
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Frontiers Media S.A.
2021-06-01
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Series: | Frontiers in Plant Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.681283/full |
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doaj-e83b3e640f254bb0bc481e9275c25f07 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xuehui Zhao Xuehui Zhao Xuehui Zhao Binbin Wen Binbin Wen Binbin Wen Chen Li Chen Li Chen Li Qiuping Tan Qiuping Tan Qiuping Tan Li Liu Xiude Chen Xiude Chen Xiude Chen Ling Li Ling Li Ling Li Xiling Fu Xiling Fu Xiling Fu |
spellingShingle |
Xuehui Zhao Xuehui Zhao Xuehui Zhao Binbin Wen Binbin Wen Binbin Wen Chen Li Chen Li Chen Li Qiuping Tan Qiuping Tan Qiuping Tan Li Liu Xiude Chen Xiude Chen Xiude Chen Ling Li Ling Li Ling Li Xiling Fu Xiling Fu Xiling Fu Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar Frontiers in Plant Science PpEBB1 shoot branching sugars amino acids brassinosteroids light response |
author_facet |
Xuehui Zhao Xuehui Zhao Xuehui Zhao Binbin Wen Binbin Wen Binbin Wen Chen Li Chen Li Chen Li Qiuping Tan Qiuping Tan Qiuping Tan Li Liu Xiude Chen Xiude Chen Xiude Chen Ling Li Ling Li Ling Li Xiling Fu Xiling Fu Xiling Fu |
author_sort |
Xuehui Zhao |
title |
Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar |
title_short |
Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar |
title_full |
Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar |
title_fullStr |
Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar |
title_full_unstemmed |
Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar |
title_sort |
overexpression of the peach transcription factor early bud-break 1 leads to more branches in poplar |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-06-01 |
description |
Shoot branching is an important adaptive trait that determines plant architecture. In a previous study, the Early bud-break 1 (EBB1) gene in peach (Prunus persica var. nectarina) cultivar Zhongyou 4 was transformed into poplar (Populus trichocarpa). PpEBB1-oe poplar showed a more branched phenotype. To understand the potential mechanisms underlying the EBB1-mediated branching, transcriptomic and proteomics analyses were used. The results showed that a large number of differentially expressed genes (DEGs)/differentially expressed proteins (DEPs) associated with light response, sugars, brassinosteroids (BR), and nitrogen metabolism were significantly enriched in PpEBB1-oe poplar. In addition, contents of sugars, BR, and amino acids were measured. Results showed that PpEBB1 significantly promoted the accumulation of fructose, glucose, sucrose, trehalose, and starch. Contents of brassinolide (BL), castasterone (CS), and 6-deoxocathasterone (6-deoxoCS) were all significantly changed with overexpressing PpEBB1. Various types of amino acids were measured and four of them were significantly improved in PpEBB1-oe poplar, including aspartic acid (Asp), arginine (Arg), cysteine (Cys), and tryptohpan (Trp). Taken together, shoot branching is a process controlled by a complex regulatory network, and PpEBB1 may play important roles in this process through the coordinating multiple metabolic pathways involved in shoot branching, including light response, phytohormones, sugars, and nitrogen. |
topic |
PpEBB1 shoot branching sugars amino acids brassinosteroids light response |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.681283/full |
work_keys_str_mv |
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doaj-e83b3e640f254bb0bc481e9275c25f072021-06-17T09:49:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.681283681283Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in PoplarXuehui Zhao0Xuehui Zhao1Xuehui Zhao2Binbin Wen3Binbin Wen4Binbin Wen5Chen Li6Chen Li7Chen Li8Qiuping Tan9Qiuping Tan10Qiuping Tan11Li Liu12Xiude Chen13Xiude Chen14Xiude Chen15Ling Li16Ling Li17Ling Li18Xiling Fu19Xiling Fu20Xiling Fu21College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaShandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, ChinaShoot branching is an important adaptive trait that determines plant architecture. In a previous study, the Early bud-break 1 (EBB1) gene in peach (Prunus persica var. nectarina) cultivar Zhongyou 4 was transformed into poplar (Populus trichocarpa). PpEBB1-oe poplar showed a more branched phenotype. To understand the potential mechanisms underlying the EBB1-mediated branching, transcriptomic and proteomics analyses were used. The results showed that a large number of differentially expressed genes (DEGs)/differentially expressed proteins (DEPs) associated with light response, sugars, brassinosteroids (BR), and nitrogen metabolism were significantly enriched in PpEBB1-oe poplar. In addition, contents of sugars, BR, and amino acids were measured. Results showed that PpEBB1 significantly promoted the accumulation of fructose, glucose, sucrose, trehalose, and starch. Contents of brassinolide (BL), castasterone (CS), and 6-deoxocathasterone (6-deoxoCS) were all significantly changed with overexpressing PpEBB1. Various types of amino acids were measured and four of them were significantly improved in PpEBB1-oe poplar, including aspartic acid (Asp), arginine (Arg), cysteine (Cys), and tryptohpan (Trp). Taken together, shoot branching is a process controlled by a complex regulatory network, and PpEBB1 may play important roles in this process through the coordinating multiple metabolic pathways involved in shoot branching, including light response, phytohormones, sugars, and nitrogen.https://www.frontiersin.org/articles/10.3389/fpls.2021.681283/fullPpEBB1shoot branchingsugarsamino acidsbrassinosteroidslight response |