A functional variant rs12904 in the miR-200c binding site was associated with a decreased risk of ischemic stroke

• Main Authors: Zhi-Neng Zeng, Ling-Ling Liu, Yong-Ling He, Xiang Shi, Ye-Sheng Wei
• Format: Article
• Language: English
• Published: BMC 2019-05-01
• Series: Lipids in Health and Disease
• Subjects: miR-200c; Genome-wide association study; Polymorphism; Ischemic stroke
• Online Access: http://link.springer.com/article/10.1186/s12944-019-1060-1
• ISSN: 1476-511X

Abstract Genome-wide association study (GWAS) identified chromosome 12p13 rs12425791 and rs11833579 as susceptibility loci of ischemic stroke (IS) in a European population. However, conflicting results were obtained in subsequent replication analysis. miR-200c, located on chromosome 12p13, was found to have a neuroprotective effect on ischemia. Our aim of this study was to investigate the association of the rs12425791, rs11833579 and rs12904 in the binding site of miR-200c with the risk of IS. The rs12425791, rs11833579, and rs12904 were genotyped using a TaqMan allelic discrimination assay. The results were verified by Sanger sequencing. We found that the rs12904 AG/GG genotypes and G allele were associated with a decreased risk of IS (AG/GG vs. AA: adjusted OR = 0.64; 95% CI, 0.44–0.95; G vs. A: adjusted OR = 0.65; 95% CI, 0.46–0.93). The combined genotypes of the rs11833579AG/AA and rs12904AG/GG were also associated with a reduced risk of IS (OR = 0.65; 95% CI, 0.46–0.93). These findings suggest that the rs12904 may have a jointly protective effect against the risk of IS.