Walnut Protein Hydrolysates Play a Protective Role on Neurotoxicity Induced by d-Galactose and Aluminum Chloride in Mice

• Main Authors: Li Feng, Xiaojing Wang, Fei Peng, Jianqiao Liao, Yifan Nai, Hongjie Lei, Mei Li, Huaide Xu
• Format: Article
• Language: English
• Published: MDPI AG 2018-09-01
• Series: Molecules
• Subjects: walnut protein hydrolysates; gastrointestinal digestion; d-galactose; aluminum chloride; neurotoxicity; antioxidant activity
• Online Access: http://www.mdpi.com/1420-3049/23/9/2308

In recent years, with an increase in the aging population, neurodegenerative diseases have attracted more and more attention. This study aimed to investigate the potential neuroprotective effect of defatted walnut meal protein hydrolysates (DWMPH) on neurotoxicity induced by d-galactose (d-gal) and aluminum chloride (AlCl3) in mice. The animal models were established by combining treatments with d-gal (200 mg/kg/day, subcutaneously) and AlCl3 (100 mg/kg in drinking water) for 90 days. During the 90 days, 1 g/kg of DWMPH was administrated orally every day. The results indicated that DWMPH treatment alleviated oxidative stress, reversed cholinergic dysfunction, and suppressed the release of proinflammatory cytokines in the brains of d-gal + AlCl3-treated mice, and thus improving the learning and memory functions of these mice, which was closely correlated with the strong antioxidant activity of DWMPH. This finding suggests that DWMPH might be a promising dietary supplement in improving neuronal dysfunctions of the brain.