TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine
T cells play an essential role in the immune response against the human respiratory syncytial virus (hRSV). It has been described that both CD4<sup>+</sup> and CD8<sup>+</sup> T cells can contribute to the clearance of the virus during an infection. However, for some individu...
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doaj-e8550436568e46658c360faa5ac70fce2020-11-25T01:19:52ZengMDPI AGViruses1999-49152020-02-0112223310.3390/v12020233v12020233TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG VaccineEmma Rey-Jurado0Karen Bohmwald1Hernán G. Correa2Alexis M. Kalergis3Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileMillennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileMillennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileMillennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileT cells play an essential role in the immune response against the human respiratory syncytial virus (hRSV). It has been described that both CD4<sup>+</sup> and CD8<sup>+</sup> T cells can contribute to the clearance of the virus during an infection. However, for some individuals, such an immune response can lead to an exacerbated and detrimental inflammatory response with high recruitment of neutrophils to the lungs. The receptor of most T cells is a heterodimer consisting of α and β chains (αβTCR) that upon antigen engagement induces the activation of these cells. The αβTCR molecule displays a broad sequence diversity that defines the T cell repertoire of an individual. In our laboratory, a recombinant Bacille Calmette–Guérin (BCG) vaccine expressing the nucleoprotein (N) of hRSV (rBCG-N-hRSV) was developed. Such a vaccine induces T cells with a Th1 polarized phenotype that promote the clearance of hRSV infection without causing inflammatory lung damage. Importantly, as part of this work, the T cell receptor (TCR) repertoire of T cells expanded after hRSV infection in naïve and rBCG-N-hRSV-immunized mice was characterized. A more diverse TCR repertoire was observed in the lungs from rBCG-N-hRSV-immunized as compared to unimmunized hRSV-infected mice, suggesting that vaccination with the recombinant rBCG-N-hRSV vaccine triggers the expansion of T cell populations that recognize more viral epitopes. Furthermore, differential expansion of certain TCRVβ chains was found for hRSV infection (TCRVβ<sup>+</sup>8.3 and TCRVβ<sup>+</sup>5.1,5.2) as compared to rBCG-N-hRSV vaccination (TCRVβ<sup>+</sup>11 and TCRVβ<sup>+</sup>12). Our findings contribute to better understanding the T cell response during hRSV infection, as well as the functioning of a vaccine that induces a protective T cell immunity against this virus.https://www.mdpi.com/1999-4915/12/2/233human respiratory syncytial virusrecombinant bcg vaccinetcr repertoire |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emma Rey-Jurado Karen Bohmwald Hernán G. Correa Alexis M. Kalergis |
spellingShingle |
Emma Rey-Jurado Karen Bohmwald Hernán G. Correa Alexis M. Kalergis TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine Viruses human respiratory syncytial virus recombinant bcg vaccine tcr repertoire |
author_facet |
Emma Rey-Jurado Karen Bohmwald Hernán G. Correa Alexis M. Kalergis |
author_sort |
Emma Rey-Jurado |
title |
TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine |
title_short |
TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine |
title_full |
TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine |
title_fullStr |
TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine |
title_full_unstemmed |
TCR Repertoire Characterization for T Cells Expanded in Response to hRSV Infection in Mice Immunized with a Recombinant BCG Vaccine |
title_sort |
tcr repertoire characterization for t cells expanded in response to hrsv infection in mice immunized with a recombinant bcg vaccine |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2020-02-01 |
description |
T cells play an essential role in the immune response against the human respiratory syncytial virus (hRSV). It has been described that both CD4<sup>+</sup> and CD8<sup>+</sup> T cells can contribute to the clearance of the virus during an infection. However, for some individuals, such an immune response can lead to an exacerbated and detrimental inflammatory response with high recruitment of neutrophils to the lungs. The receptor of most T cells is a heterodimer consisting of α and β chains (αβTCR) that upon antigen engagement induces the activation of these cells. The αβTCR molecule displays a broad sequence diversity that defines the T cell repertoire of an individual. In our laboratory, a recombinant Bacille Calmette–Guérin (BCG) vaccine expressing the nucleoprotein (N) of hRSV (rBCG-N-hRSV) was developed. Such a vaccine induces T cells with a Th1 polarized phenotype that promote the clearance of hRSV infection without causing inflammatory lung damage. Importantly, as part of this work, the T cell receptor (TCR) repertoire of T cells expanded after hRSV infection in naïve and rBCG-N-hRSV-immunized mice was characterized. A more diverse TCR repertoire was observed in the lungs from rBCG-N-hRSV-immunized as compared to unimmunized hRSV-infected mice, suggesting that vaccination with the recombinant rBCG-N-hRSV vaccine triggers the expansion of T cell populations that recognize more viral epitopes. Furthermore, differential expansion of certain TCRVβ chains was found for hRSV infection (TCRVβ<sup>+</sup>8.3 and TCRVβ<sup>+</sup>5.1,5.2) as compared to rBCG-N-hRSV vaccination (TCRVβ<sup>+</sup>11 and TCRVβ<sup>+</sup>12). Our findings contribute to better understanding the T cell response during hRSV infection, as well as the functioning of a vaccine that induces a protective T cell immunity against this virus. |
topic |
human respiratory syncytial virus recombinant bcg vaccine tcr repertoire |
url |
https://www.mdpi.com/1999-4915/12/2/233 |
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