Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane
We have investigated the anticancer effects of the dietary isothiocyanate sulforaphane (SFN) on colorectal cancer (CRC), using primary cancer cells lines isolated from five Taiwanese colorectal cancer patients as the model for colorectal cancer. SFN-treated cells accumulated in metaphase (SFN 6.25 μ...
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doaj-e87cc7283c754193bf72e874375471562020-11-25T01:22:39ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/415231415231Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by SulforaphaneMing-Jenn Chen0Wei-Yu Tang1Che-Wei Hsu2Ya-Ting Tsai3June-Fu Wu4Chen-Wei Lin5Ya-Min Cheng6Yi-Chiang Hsu7Division of Traumatology, Department of Surgery, Chi Mei Medical Center, Tainan, TaiwanGraduate Institute of Medical Science, College of Health Sciences, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren District, Tainan City 71101, TaiwanDepartment of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren District, Tainan City 71101, TaiwanGraduate Institute of Medical Science, College of Health Sciences, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren District, Tainan City 71101, TaiwanDepartment of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren District, Tainan City 71101, TaiwanDepartment of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren District, Tainan City 71101, TaiwanDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, TaiwanGraduate Institute of Medical Science, College of Health Sciences, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren District, Tainan City 71101, TaiwanWe have investigated the anticancer effects of the dietary isothiocyanate sulforaphane (SFN) on colorectal cancer (CRC), using primary cancer cells lines isolated from five Taiwanese colorectal cancer patients as the model for colorectal cancer. SFN-treated cells accumulated in metaphase (SFN 6.25 μM) and subG1 (SFN 12.5 and 25 μM) as determined by flow cytometry. In addition, treated cells showed nuclear apoptotic morphology that coincided with an activation of caspase-3, and loss of mitochondrial membrane potential (ΔΨm). Incubations at higher SFN doses (12.5 and 25 μM) resulted in cleavage of procaspase-3 and elevated caspase-2, -3, -8, and -9 activity, suggesting that the induction of apoptosis and the sulforaphane-induced mitosis delay at the lower dose are independently regulated. Daily SFN s.c. injections (400 micromol/kg/d for 3 weeks) in severe combined immunodeficient mice with primary human CRC (CP1 to CP5) s.c. tumors resulted in a decrease of mean tumor weight by 70% compared with vehicle-treated controls. Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have antitumor activity in established colorectal cancer.http://dx.doi.org/10.1155/2012/415231 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming-Jenn Chen Wei-Yu Tang Che-Wei Hsu Ya-Ting Tsai June-Fu Wu Chen-Wei Lin Ya-Min Cheng Yi-Chiang Hsu |
spellingShingle |
Ming-Jenn Chen Wei-Yu Tang Che-Wei Hsu Ya-Ting Tsai June-Fu Wu Chen-Wei Lin Ya-Min Cheng Yi-Chiang Hsu Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane Evidence-Based Complementary and Alternative Medicine |
author_facet |
Ming-Jenn Chen Wei-Yu Tang Che-Wei Hsu Ya-Ting Tsai June-Fu Wu Chen-Wei Lin Ya-Min Cheng Yi-Chiang Hsu |
author_sort |
Ming-Jenn Chen |
title |
Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane |
title_short |
Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane |
title_full |
Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane |
title_fullStr |
Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane |
title_full_unstemmed |
Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane |
title_sort |
apoptosis induction in primary human colorectal cancer cell lines and retarded tumor growth in scid mice by sulforaphane |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
We have investigated the anticancer effects of the dietary isothiocyanate sulforaphane (SFN) on colorectal cancer (CRC), using primary cancer cells lines isolated from five Taiwanese colorectal cancer patients as the model for colorectal cancer. SFN-treated cells accumulated in metaphase (SFN 6.25 μM) and subG1 (SFN 12.5 and 25 μM) as determined by flow cytometry. In addition, treated cells showed nuclear apoptotic morphology that coincided with an activation of caspase-3, and loss of mitochondrial membrane potential (ΔΨm). Incubations at higher SFN doses (12.5 and 25 μM) resulted in cleavage of procaspase-3 and elevated caspase-2, -3, -8, and -9 activity, suggesting that the induction of apoptosis and the sulforaphane-induced mitosis delay at the lower dose are independently regulated. Daily SFN s.c. injections (400 micromol/kg/d for 3 weeks) in severe combined immunodeficient mice with primary human CRC (CP1 to CP5) s.c. tumors resulted in a decrease of mean tumor weight by 70% compared with vehicle-treated controls. Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have antitumor activity in established colorectal cancer. |
url |
http://dx.doi.org/10.1155/2012/415231 |
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