Protein kinase C is a calcium sensor for presynaptic short-term plasticity
In presynaptic boutons, calcium (Ca2+) triggers both neurotransmitter release and short-term synaptic plasticity. Whereas synaptotagmins are known to mediate vesicle fusion through binding of high local Ca2+ to their C2 domains, the proteins that sense smaller global Ca2+ increases to produce short-...
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doaj-e89d25fd753a43cb934e13dd26a133962021-05-04T23:24:22ZengeLife Sciences Publications LtdeLife2050-084X2014-08-01310.7554/eLife.03011Protein kinase C is a calcium sensor for presynaptic short-term plasticityDiasynou Fioravante0YunXiang Chu1Arthur PH de Jong2Michael Leitges3Pascal S Kaeser4Wade G Regehr5Department of Neurobiology, Harvard Medical School, Boston, United States; Center for Neuroscience, University of California, Davis, Davis, United StatesDepartment of Neurobiology, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Harvard Medical School, Boston, United StatesThe Biotechnology Center of Oslo, University of Oslo, Oslo, NorwayDepartment of Neurobiology, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Harvard Medical School, Boston, United StatesIn presynaptic boutons, calcium (Ca2+) triggers both neurotransmitter release and short-term synaptic plasticity. Whereas synaptotagmins are known to mediate vesicle fusion through binding of high local Ca2+ to their C2 domains, the proteins that sense smaller global Ca2+ increases to produce short-term plasticity have remained elusive. Here, we identify a Ca2+ sensor for post-tetanic potentiation (PTP), a form of plasticity thought to underlie short-term memory. We find that at the functionally mature calyx of Held synapse the Ca2+-dependent protein kinase C isoforms α and β are necessary for PTP, and the expression of PKCβ in PKCαβ double knockout mice rescues PTP. Disruption of Ca2+ binding to the PKCβ C2 domain specifically prevents PTP without impairing other PKCβ-dependent forms of synaptic enhancement. We conclude that different C2-domain-containing presynaptic proteins are engaged by different Ca2+ signals, and that Ca2+ increases evoked by tetanic stimulation are sensed by PKCβ to produce PTP.https://elifesciences.org/articles/03011post-tetanic potentiationshort-term plasticityprotein kinase Csynaptotagminphorbol estercalcium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Diasynou Fioravante YunXiang Chu Arthur PH de Jong Michael Leitges Pascal S Kaeser Wade G Regehr |
spellingShingle |
Diasynou Fioravante YunXiang Chu Arthur PH de Jong Michael Leitges Pascal S Kaeser Wade G Regehr Protein kinase C is a calcium sensor for presynaptic short-term plasticity eLife post-tetanic potentiation short-term plasticity protein kinase C synaptotagmin phorbol ester calcium |
author_facet |
Diasynou Fioravante YunXiang Chu Arthur PH de Jong Michael Leitges Pascal S Kaeser Wade G Regehr |
author_sort |
Diasynou Fioravante |
title |
Protein kinase C is a calcium sensor for presynaptic short-term plasticity |
title_short |
Protein kinase C is a calcium sensor for presynaptic short-term plasticity |
title_full |
Protein kinase C is a calcium sensor for presynaptic short-term plasticity |
title_fullStr |
Protein kinase C is a calcium sensor for presynaptic short-term plasticity |
title_full_unstemmed |
Protein kinase C is a calcium sensor for presynaptic short-term plasticity |
title_sort |
protein kinase c is a calcium sensor for presynaptic short-term plasticity |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2014-08-01 |
description |
In presynaptic boutons, calcium (Ca2+) triggers both neurotransmitter release and short-term synaptic plasticity. Whereas synaptotagmins are known to mediate vesicle fusion through binding of high local Ca2+ to their C2 domains, the proteins that sense smaller global Ca2+ increases to produce short-term plasticity have remained elusive. Here, we identify a Ca2+ sensor for post-tetanic potentiation (PTP), a form of plasticity thought to underlie short-term memory. We find that at the functionally mature calyx of Held synapse the Ca2+-dependent protein kinase C isoforms α and β are necessary for PTP, and the expression of PKCβ in PKCαβ double knockout mice rescues PTP. Disruption of Ca2+ binding to the PKCβ C2 domain specifically prevents PTP without impairing other PKCβ-dependent forms of synaptic enhancement. We conclude that different C2-domain-containing presynaptic proteins are engaged by different Ca2+ signals, and that Ca2+ increases evoked by tetanic stimulation are sensed by PKCβ to produce PTP. |
topic |
post-tetanic potentiation short-term plasticity protein kinase C synaptotagmin phorbol ester calcium |
url |
https://elifesciences.org/articles/03011 |
work_keys_str_mv |
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