The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis
Background: Multiple sclerosis (MS) is an autoimmune disease whereby myelin sheath of the central nervous system is destroyed. Vitamin A is known to play a role in the immune system. It has been recognized that some metabolites of vitamin A can be used effectively to treat experimental autoimmune en...
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2012-09-01
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doaj-e89f1e5e292f438e8441999ef9d114402021-04-02T12:22:26ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Neurosciences in Rural Practice0976-31470976-31552012-09-01030329429810.4103/0976-3147.102609The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosisSima Jafarirad0Fereydoon Siassi1Mohammad-Hossein Harirchian2Mohammad-Ali Sahraian3Mohammad-Reza Eshraghian4Fazel Shokri5Reza Amani6Sama Bitarafan7Sabah Mozafari8Aliakbar Saboor-Yaraghi9Department of Nutrition and Biochemistry, Tehran University of Medical Sciences, TehranDepartment of Nutrition and Biochemistry, Tehran University of Medical Sciences, TehranDepartment of Neurology, Iranian Center for Neurological Research, Tehran University of Medical Sciences, TehranDepartment of Neurology, Sina Hospital, Tehran University of Medical Sciences, TehranDepartment of Biostatistics, Tehran University of Medical Sciences, TehranDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences, TehranDepartment of Nutrition, School of Paramedicine, Jondi-Shapour University of Medical Sciences, Ahvaz, IranDepartment of Nutrition and Biochemistry, Tehran University of Medical Sciences, TehranUniversité Pierre et Marie Curie, Centre de Recherche de l’Institut du Cerveau et de la Moelle Epinière, Paris, FranceDepartment of Nutrition and Biochemistry, Tehran University of Medical Sciences, TehranBackground: Multiple sclerosis (MS) is an autoimmune disease whereby myelin sheath of the central nervous system is destroyed. Vitamin A is known to play a role in the immune system. It has been recognized that some metabolites of vitamin A can be used effectively to treat experimental autoimmune encephalomyelitis (EAE). Aims: The effect of vitamin A as retinyl palmitate on T-cell proliferation in MS patients. Setting and Design: This study is a double blind clinical trial of two test groups over a period of 6 months. Materials and Methods: Thirty five multiple sclerosis (MS) patients were divided into two groups. One group received 25,000 IU/day vitamin A (as retinyl palmitate) and the other group were administered a placebo. The peripheral blood mononuclear cells (PBMCs) were separated and stimulated with myelin oligodendrocyte glycoprotein (MOG) and phytohemagglutinin (PHA) before and after the trial period. BrdU calorimetric assay was performed to measure cell proliferation. Statistical Analysis: Analysis of covariance (ANCOVA) and paired t-test were used to analyze the data. Results: Observations showed statistical significant differences in the reduction of cell proliferation in the presence of MOG and fetal calf serum (FCS) in the culture medium, between patients receiving vitamin A and the placebo (P = 0.046). Although, this difference was not significant between the two vitamin A and placebo groups in MOG treatment with human serum, a decrease was observed in the group of patients taking vitamin A supplements (P = 0.019). Phytohemagglutinin did not cause any change in cell proliferation between the two groups. Conclusion: The results suggest supplementation with retinyl palmitate in patients with MS reduce MOG stimulatory effects on T-cells.http://www.thieme-connect.de/DOI/DOI?10.4103/0976-3147.102609cell proliferationmultiple sclerosismyelin oligodendrocyte glycoproteinvitamin a |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sima Jafarirad Fereydoon Siassi Mohammad-Hossein Harirchian Mohammad-Ali Sahraian Mohammad-Reza Eshraghian Fazel Shokri Reza Amani Sama Bitarafan Sabah Mozafari Aliakbar Saboor-Yaraghi |
spellingShingle |
Sima Jafarirad Fereydoon Siassi Mohammad-Hossein Harirchian Mohammad-Ali Sahraian Mohammad-Reza Eshraghian Fazel Shokri Reza Amani Sama Bitarafan Sabah Mozafari Aliakbar Saboor-Yaraghi The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis Journal of Neurosciences in Rural Practice cell proliferation multiple sclerosis myelin oligodendrocyte glycoprotein vitamin a |
author_facet |
Sima Jafarirad Fereydoon Siassi Mohammad-Hossein Harirchian Mohammad-Ali Sahraian Mohammad-Reza Eshraghian Fazel Shokri Reza Amani Sama Bitarafan Sabah Mozafari Aliakbar Saboor-Yaraghi |
author_sort |
Sima Jafarirad |
title |
The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis |
title_short |
The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis |
title_full |
The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis |
title_fullStr |
The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis |
title_full_unstemmed |
The effect of vitamin A supplementation on stimulated T-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis |
title_sort |
effect of vitamin a supplementation on stimulated t-cell proliferation with myelin oligodendrocyte glycoprotein in patients with multiple sclerosis |
publisher |
Thieme Medical and Scientific Publishers Pvt. Ltd. |
series |
Journal of Neurosciences in Rural Practice |
issn |
0976-3147 0976-3155 |
publishDate |
2012-09-01 |
description |
Background: Multiple sclerosis (MS) is an autoimmune disease whereby myelin sheath of the central nervous system is destroyed. Vitamin A is known to play a role in the immune system. It has been recognized that some metabolites of vitamin A can be used effectively to treat experimental autoimmune encephalomyelitis (EAE). Aims: The effect of vitamin A as retinyl palmitate on T-cell proliferation in MS patients. Setting and Design: This study is a double blind clinical trial of two test groups over a period of 6 months. Materials and Methods: Thirty five multiple sclerosis (MS) patients were divided into two groups. One group received 25,000 IU/day vitamin A (as retinyl palmitate) and the other group were administered a placebo. The peripheral blood mononuclear cells (PBMCs) were separated and stimulated with myelin oligodendrocyte glycoprotein (MOG) and phytohemagglutinin (PHA) before and after the trial period. BrdU calorimetric assay was performed to measure cell proliferation. Statistical Analysis: Analysis of covariance (ANCOVA) and paired t-test were used to analyze the data. Results: Observations showed statistical significant differences in the reduction of cell proliferation in the presence of MOG and fetal calf serum (FCS) in the culture medium, between patients receiving vitamin A and the placebo (P = 0.046). Although, this difference was not significant between the two vitamin A and placebo groups in MOG treatment with human serum, a decrease was observed in the group of patients taking vitamin A supplements (P = 0.019). Phytohemagglutinin did not cause any change in cell proliferation between the two groups. Conclusion: The results suggest supplementation with retinyl palmitate in patients with MS reduce MOG stimulatory effects on T-cells. |
topic |
cell proliferation multiple sclerosis myelin oligodendrocyte glycoprotein vitamin a |
url |
http://www.thieme-connect.de/DOI/DOI?10.4103/0976-3147.102609 |
work_keys_str_mv |
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