Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electr...
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doaj-e8aaf757327947f382eef0de9fc5453d2021-02-14T00:03:11ZengMDPI AGSensors1424-82202021-02-01211334133410.3390/s21041334Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary ElectrophoresisJin Chen0Yu Sun1Xiaogai Peng2Yi Ni3Fengchao Wang4Xiaoming Dou5College of Sciences, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, ChinaCollege of Sciences, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, ChinaCollege of Sciences, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, ChinaInstitute of Photonics and Bio-Medicine, Graduate School of Science, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, ChinaCollege of Sciences, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, ChinaInstitute of Photonics and Bio-Medicine, Graduate School of Science, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electrophoresis (microchip CE) system was employed for the rapid determination of <i>S. aureus</i>, while a simple-to-use space domain internal standard (SDIS) method was carried out for the reliable quantitative analysis. The precision, accuracy, and reliability of SDIS were investigated in detail. Noted that these properties could be elevated in SDIS compared with traditional IS method. Remarkably, the PCR products of <i>S. aureus</i><i>nuc</i> gene could be identified and quantitated within 80 s. The theoretical detection limit could achieve a value of 0.066 ng/μL, determined by the using SDIS method. The current work may provide a promising detection strategy for the high-speed and highly efficient analysis of pathogens in the fields of food safety and clinical diagnosis.https://www.mdpi.com/1424-8220/21/4/1334<i>Staphylococcus aureus</i><i>nuc</i> genemicrochip capillary electrophoresisspace domain internal standard method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Chen Yu Sun Xiaogai Peng Yi Ni Fengchao Wang Xiaoming Dou |
spellingShingle |
Jin Chen Yu Sun Xiaogai Peng Yi Ni Fengchao Wang Xiaoming Dou Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis Sensors <i>Staphylococcus aureus</i> <i>nuc</i> gene microchip capillary electrophoresis space domain internal standard method |
author_facet |
Jin Chen Yu Sun Xiaogai Peng Yi Ni Fengchao Wang Xiaoming Dou |
author_sort |
Jin Chen |
title |
Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis |
title_short |
Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis |
title_full |
Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis |
title_fullStr |
Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis |
title_full_unstemmed |
Rapid Analysis for <i>Staphylococcus aureus</i> via Microchip Capillary Electrophoresis |
title_sort |
rapid analysis for <i>staphylococcus aureus</i> via microchip capillary electrophoresis |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2021-02-01 |
description |
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electrophoresis (microchip CE) system was employed for the rapid determination of <i>S. aureus</i>, while a simple-to-use space domain internal standard (SDIS) method was carried out for the reliable quantitative analysis. The precision, accuracy, and reliability of SDIS were investigated in detail. Noted that these properties could be elevated in SDIS compared with traditional IS method. Remarkably, the PCR products of <i>S. aureus</i><i>nuc</i> gene could be identified and quantitated within 80 s. The theoretical detection limit could achieve a value of 0.066 ng/μL, determined by the using SDIS method. The current work may provide a promising detection strategy for the high-speed and highly efficient analysis of pathogens in the fields of food safety and clinical diagnosis. |
topic |
<i>Staphylococcus aureus</i> <i>nuc</i> gene microchip capillary electrophoresis space domain internal standard method |
url |
https://www.mdpi.com/1424-8220/21/4/1334 |
work_keys_str_mv |
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