Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients
Abstract Background Low muscle radiodensity is associated with mortality in a variety of cancer types. Biochemical and morphological correlates are unknown. We aimed to evaluate triglyceride (TG) content and location as a function of computed tomography (CT)‐derived measures of skeletal muscle radio...
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doaj-e8aba141f2194e42aa44b835c0d939252020-11-25T03:06:13ZengWileyJournal of Cachexia, Sarcopenia and Muscle2190-59912190-60092020-06-0111373574710.1002/jcsm.12533Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patientsAmritpal S. Bhullar0Ana Anoveros‐Barrera1Abha Dunichand‐Hoedl2Karen Martins3David Bigam4Rachel G. Khadaroo5Todd McMullen6Oliver F. Bathe7Charles T. Putman8Michael T. Clandinin9Vickie E. Baracos10Vera C. Mazurak11Department of Agricultural, Food & Nutritional Science University of Alberta 4‐002 Li Ka Shing Centre for Health Research Innovation Edmonton Alberta CanadaDepartment of Agricultural, Food & Nutritional Science University of Alberta 4‐002 Li Ka Shing Centre for Health Research Innovation Edmonton Alberta CanadaDepartment of Agricultural, Food & Nutritional Science University of Alberta 4‐002 Li Ka Shing Centre for Health Research Innovation Edmonton Alberta CanadaDepartment of Agricultural, Food & Nutritional Science University of Alberta 4‐002 Li Ka Shing Centre for Health Research Innovation Edmonton Alberta CanadaDepartment of Surgery University of Alberta Edmonton CanadaDepartment of Surgery University of Alberta Edmonton CanadaDepartment of Surgery University of Alberta Edmonton CanadaDepartments of Surgery and Oncology, Tom Baker Cancer Centre University of Calgary Calgary CanadaFaculty of Kinesiology, Sport, and Recreation University of Alberta Edmonton CanadaDepartment of Agricultural, Food & Nutritional Science University of Alberta 4‐002 Li Ka Shing Centre for Health Research Innovation Edmonton Alberta CanadaDepartment of Oncology University of Alberta Edmonton CanadaDepartment of Agricultural, Food & Nutritional Science University of Alberta 4‐002 Li Ka Shing Centre for Health Research Innovation Edmonton Alberta CanadaAbstract Background Low muscle radiodensity is associated with mortality in a variety of cancer types. Biochemical and morphological correlates are unknown. We aimed to evaluate triglyceride (TG) content and location as a function of computed tomography (CT)‐derived measures of skeletal muscle radiodensity in cancer patients. Methods Rectus abdominis (RA) biopsies were collected during cancer surgery from 75 patients diagnosed with cancer. Thin‐layer chromatography and gas chromatography were used for quantification of TG content of the muscle. Axial CT images of lumbar vertebra were used to measure muscle radiodensity. Oil Red O staining was used to determine the location of neutral lipids in frozen muscle sections. Results There was wide variation in RA radiodensity in repeated measures (CV% ranged from 3 to 55% based on 10 serial images) as well as within one slice (CV% ranged from 6 to 61% based on 10 subregions). RA radiodensity and total lumbar muscle radiodensity were inversely associated with TG content of RA (r = −0.396, P < 0.001, and r = −0.355, P = 0.002, respectively). Of the total percentage area of muscle staining positive for neutral lipid, 54 ± 17% was present as extramyocellular lipids (range 23.5–77.8%) and 46 ± 17% (range 22.2–76.5%) present as intramyocellular lipid droplets. Conclusions Repeated measures revealed wide variation in radiodensity of RA muscle, both vertically and horizontally. Low muscle radiodensity reflects high level of TG in patients with cancer. Non‐uniform distribution of intramyocellular and extramyocellular lipids was evident using light microscopy. These results warrant investigation of mechanisms resulting in lipid deposition in muscles of cancer patients.https://doi.org/10.1002/jcsm.12533Fat infiltrationHounsfield unitsMuscle attenuationMyosteatosisRectus abdominisSkeletal muscle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amritpal S. Bhullar Ana Anoveros‐Barrera Abha Dunichand‐Hoedl Karen Martins David Bigam Rachel G. Khadaroo Todd McMullen Oliver F. Bathe Charles T. Putman Michael T. Clandinin Vickie E. Baracos Vera C. Mazurak |
spellingShingle |
Amritpal S. Bhullar Ana Anoveros‐Barrera Abha Dunichand‐Hoedl Karen Martins David Bigam Rachel G. Khadaroo Todd McMullen Oliver F. Bathe Charles T. Putman Michael T. Clandinin Vickie E. Baracos Vera C. Mazurak Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients Journal of Cachexia, Sarcopenia and Muscle Fat infiltration Hounsfield units Muscle attenuation Myosteatosis Rectus abdominis Skeletal muscle |
author_facet |
Amritpal S. Bhullar Ana Anoveros‐Barrera Abha Dunichand‐Hoedl Karen Martins David Bigam Rachel G. Khadaroo Todd McMullen Oliver F. Bathe Charles T. Putman Michael T. Clandinin Vickie E. Baracos Vera C. Mazurak |
author_sort |
Amritpal S. Bhullar |
title |
Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients |
title_short |
Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients |
title_full |
Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients |
title_fullStr |
Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients |
title_full_unstemmed |
Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients |
title_sort |
lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients |
publisher |
Wiley |
series |
Journal of Cachexia, Sarcopenia and Muscle |
issn |
2190-5991 2190-6009 |
publishDate |
2020-06-01 |
description |
Abstract Background Low muscle radiodensity is associated with mortality in a variety of cancer types. Biochemical and morphological correlates are unknown. We aimed to evaluate triglyceride (TG) content and location as a function of computed tomography (CT)‐derived measures of skeletal muscle radiodensity in cancer patients. Methods Rectus abdominis (RA) biopsies were collected during cancer surgery from 75 patients diagnosed with cancer. Thin‐layer chromatography and gas chromatography were used for quantification of TG content of the muscle. Axial CT images of lumbar vertebra were used to measure muscle radiodensity. Oil Red O staining was used to determine the location of neutral lipids in frozen muscle sections. Results There was wide variation in RA radiodensity in repeated measures (CV% ranged from 3 to 55% based on 10 serial images) as well as within one slice (CV% ranged from 6 to 61% based on 10 subregions). RA radiodensity and total lumbar muscle radiodensity were inversely associated with TG content of RA (r = −0.396, P < 0.001, and r = −0.355, P = 0.002, respectively). Of the total percentage area of muscle staining positive for neutral lipid, 54 ± 17% was present as extramyocellular lipids (range 23.5–77.8%) and 46 ± 17% (range 22.2–76.5%) present as intramyocellular lipid droplets. Conclusions Repeated measures revealed wide variation in radiodensity of RA muscle, both vertically and horizontally. Low muscle radiodensity reflects high level of TG in patients with cancer. Non‐uniform distribution of intramyocellular and extramyocellular lipids was evident using light microscopy. These results warrant investigation of mechanisms resulting in lipid deposition in muscles of cancer patients. |
topic |
Fat infiltration Hounsfield units Muscle attenuation Myosteatosis Rectus abdominis Skeletal muscle |
url |
https://doi.org/10.1002/jcsm.12533 |
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