Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins
Multiple synaptic adhesion molecules govern synapse formation. Here, we propose calsyntenin-3/alcadein-β as a synapse organizer that specifically induces presynaptic differentiation in heterologous synapse-formation assays. Calsyntenin-3 (CST-3) is highly expressed during various postnatal periods o...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2014-03-01
|
Series: | Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714001107 |
id |
doaj-e8c0fa91125c4ac08f7dec46af07b9b6 |
---|---|
record_format |
Article |
spelling |
doaj-e8c0fa91125c4ac08f7dec46af07b9b62020-11-24T21:36:16ZengElsevierCell Reports2211-12472014-03-01661096110910.1016/j.celrep.2014.02.010Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with NeurexinsJi Won Um0Gopal Pramanik1Ji Seung Ko2Min-Young Song3Dongmin Lee4Hyun Kim5Kang-Sik Park6Thomas C. Südhof7Katsuhiko Tabuchi8Jaewon Ko9Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, KoreaDepartment of Molecular and Cellular Physiology, Shinshu University School of Medicine, Matsumoto 390-8621, JapanDepartment of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, KoreaDepartment of Physiology and Neuroscience, Kyung Hee University School of Medicine, Seoul 130-701, KoreaDepartment of Anatomy and Neuroscience, Korea 21 Biomedical Science, College of Medicine, Korea University, 126-1, 5-ka, Anam-dong, Seongbuk-gu, Seoul 136-705, KoreaDepartment of Anatomy and Neuroscience, Korea 21 Biomedical Science, College of Medicine, Korea University, 126-1, 5-ka, Anam-dong, Seongbuk-gu, Seoul 136-705, KoreaDepartment of Physiology and Neuroscience, Kyung Hee University School of Medicine, Seoul 130-701, KoreaDepartment of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Molecular and Cellular Physiology, Shinshu University School of Medicine, Matsumoto 390-8621, JapanDepartment of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, KoreaMultiple synaptic adhesion molecules govern synapse formation. Here, we propose calsyntenin-3/alcadein-β as a synapse organizer that specifically induces presynaptic differentiation in heterologous synapse-formation assays. Calsyntenin-3 (CST-3) is highly expressed during various postnatal periods of mouse brain development. The simultaneous knockdown of all three CSTs, but not CST-3 alone, decreases inhibitory, but not excitatory, synapse densities in cultured hippocampal neurons. Moreover, the knockdown of CSTs specifically reduces inhibitory synaptic transmission in vitro and in vivo. Remarkably, the loss of CSTs induces a concomitant decrease in neuron soma size in a non-cell-autonomous manner. Furthermore, α-neurexins (α-Nrxs) are components of a CST-3 complex involved in CST-3-mediated presynaptic differentiation. However, CST-3 does not directly bind to Nrxs. Viewed together, these data suggest that the three CSTs redundantly regulate inhibitory synapse formation, inhibitory synapse function, and neuron development in concert with Nrxs.http://www.sciencedirect.com/science/article/pii/S2211124714001107 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ji Won Um Gopal Pramanik Ji Seung Ko Min-Young Song Dongmin Lee Hyun Kim Kang-Sik Park Thomas C. Südhof Katsuhiko Tabuchi Jaewon Ko |
spellingShingle |
Ji Won Um Gopal Pramanik Ji Seung Ko Min-Young Song Dongmin Lee Hyun Kim Kang-Sik Park Thomas C. Südhof Katsuhiko Tabuchi Jaewon Ko Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins Cell Reports |
author_facet |
Ji Won Um Gopal Pramanik Ji Seung Ko Min-Young Song Dongmin Lee Hyun Kim Kang-Sik Park Thomas C. Südhof Katsuhiko Tabuchi Jaewon Ko |
author_sort |
Ji Won Um |
title |
Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins |
title_short |
Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins |
title_full |
Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins |
title_fullStr |
Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins |
title_full_unstemmed |
Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins |
title_sort |
calsyntenins function as synaptogenic adhesion molecules in concert with neurexins |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2014-03-01 |
description |
Multiple synaptic adhesion molecules govern synapse formation. Here, we propose calsyntenin-3/alcadein-β as a synapse organizer that specifically induces presynaptic differentiation in heterologous synapse-formation assays. Calsyntenin-3 (CST-3) is highly expressed during various postnatal periods of mouse brain development. The simultaneous knockdown of all three CSTs, but not CST-3 alone, decreases inhibitory, but not excitatory, synapse densities in cultured hippocampal neurons. Moreover, the knockdown of CSTs specifically reduces inhibitory synaptic transmission in vitro and in vivo. Remarkably, the loss of CSTs induces a concomitant decrease in neuron soma size in a non-cell-autonomous manner. Furthermore, α-neurexins (α-Nrxs) are components of a CST-3 complex involved in CST-3-mediated presynaptic differentiation. However, CST-3 does not directly bind to Nrxs. Viewed together, these data suggest that the three CSTs redundantly regulate inhibitory synapse formation, inhibitory synapse function, and neuron development in concert with Nrxs. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124714001107 |
work_keys_str_mv |
AT jiwonum calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT gopalpramanik calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT jiseungko calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT minyoungsong calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT dongminlee calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT hyunkim calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT kangsikpark calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT thomascsudhof calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT katsuhikotabuchi calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins AT jaewonko calsynteninsfunctionassynaptogenicadhesionmoleculesinconcertwithneurexins |
_version_ |
1725942021828378624 |