lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling
lncRNAs are related to the progression of various diseases, including oral squamous cell carcinoma (OSCC), which is a common squamous cell carcinoma of the head and neck. Tumor-associated macrophages and tumor cells are significant components of tumor microenvironment. M2 polarization of tumor-assoc...
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Series: | Journal of Immunology Research |
Online Access: | http://dx.doi.org/10.1155/2021/5524231 |
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doaj-e8c5856e64fb4eab88ba0d3638c673472021-08-23T01:31:59ZengHindawi LimitedJournal of Immunology Research2314-71562021-01-01202110.1155/2021/5524231lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB SignalingYilong Ai0Shiwei Liu1Hailing Luo2Siyuan Wu3Haigang Wei4Zhe Tang5Xia Li6Chen Zou7Foshan Stomatological HospitalDepartment of StomatologyFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitallncRNAs are related to the progression of various diseases, including oral squamous cell carcinoma (OSCC), which is a common squamous cell carcinoma of the head and neck. Tumor-associated macrophages and tumor cells are significant components of tumor microenvironment. M2 polarization of tumor-associated macrophages is a crucial actor in tumor malignancy and metastasis. In this study, we studied the molecular mechanism of lncRNA DCST1-AS1 in OSCC. Here, we reported that DCST1-AS1 was significantly increased in OSCC cells. We found that loss of DCST1-AS1 obviously inhibited the proliferation, migration, and invasion of OSCC cells and xenograft tumor growth. Meanwhile, silencing of DCST1-AS1 also repressed the percentage of macrophages expressing M2 markers CD206 and CD11b. DCST1-AS1 shRNA enhanced the percentage of macrophages expressing M1 markers CD80 and CD11c. Then, we observed that loss of DCST1-AS1 suppressed OSCC progression via inactivating NF-κB signaling. As well established, NF-κB signaling exerts critical roles in tumor progression, and our study proved that DCST1-AS1 could regulate NF-κB signaling. We proved that blocking the NF-κB pathway using antagonists greatly downregulated OSCC progression and M2 macrophage polarization induced by the overexpression of DCST1-AS1. To sum up, we reported that DCST1-AS1 plays an important role in modulating OSCC tumorigenicity and M2 macrophage polarization through regulating the NF-κB pathway.http://dx.doi.org/10.1155/2021/5524231 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yilong Ai Shiwei Liu Hailing Luo Siyuan Wu Haigang Wei Zhe Tang Xia Li Chen Zou |
spellingShingle |
Yilong Ai Shiwei Liu Hailing Luo Siyuan Wu Haigang Wei Zhe Tang Xia Li Chen Zou lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling Journal of Immunology Research |
author_facet |
Yilong Ai Shiwei Liu Hailing Luo Siyuan Wu Haigang Wei Zhe Tang Xia Li Chen Zou |
author_sort |
Yilong Ai |
title |
lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling |
title_short |
lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling |
title_full |
lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling |
title_fullStr |
lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling |
title_full_unstemmed |
lncRNA DCST1-AS1 Facilitates Oral Squamous Cell Carcinoma by Promoting M2 Macrophage Polarization through Activating NF-κB Signaling |
title_sort |
lncrna dcst1-as1 facilitates oral squamous cell carcinoma by promoting m2 macrophage polarization through activating nf-κb signaling |
publisher |
Hindawi Limited |
series |
Journal of Immunology Research |
issn |
2314-7156 |
publishDate |
2021-01-01 |
description |
lncRNAs are related to the progression of various diseases, including oral squamous cell carcinoma (OSCC), which is a common squamous cell carcinoma of the head and neck. Tumor-associated macrophages and tumor cells are significant components of tumor microenvironment. M2 polarization of tumor-associated macrophages is a crucial actor in tumor malignancy and metastasis. In this study, we studied the molecular mechanism of lncRNA DCST1-AS1 in OSCC. Here, we reported that DCST1-AS1 was significantly increased in OSCC cells. We found that loss of DCST1-AS1 obviously inhibited the proliferation, migration, and invasion of OSCC cells and xenograft tumor growth. Meanwhile, silencing of DCST1-AS1 also repressed the percentage of macrophages expressing M2 markers CD206 and CD11b. DCST1-AS1 shRNA enhanced the percentage of macrophages expressing M1 markers CD80 and CD11c. Then, we observed that loss of DCST1-AS1 suppressed OSCC progression via inactivating NF-κB signaling. As well established, NF-κB signaling exerts critical roles in tumor progression, and our study proved that DCST1-AS1 could regulate NF-κB signaling. We proved that blocking the NF-κB pathway using antagonists greatly downregulated OSCC progression and M2 macrophage polarization induced by the overexpression of DCST1-AS1. To sum up, we reported that DCST1-AS1 plays an important role in modulating OSCC tumorigenicity and M2 macrophage polarization through regulating the NF-κB pathway. |
url |
http://dx.doi.org/10.1155/2021/5524231 |
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