Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China
Labyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, their lipid profiles are poorly described because only a few large-scale isolation attempts have been made so far. Here, we isolated more than 200 strains from mangrove habita...
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doaj-e8d07618b2c04304abbe0abf6773a06a2020-11-24T20:46:44ZengMDPI AGMarine Drugs1660-33972019-05-0117526810.3390/md17050268md17050268Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of ChinaQiuzhen Wang0Huike Ye1Yunxuan Xie2Yaodong He3Biswarup Sen4Guangyi Wang5Center for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaLabyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, their lipid profiles are poorly described because only a few large-scale isolation attempts have been made so far. Here, we isolated more than 200 strains from mangrove habitats of China and characterized the molecular phylogeny and lipid accumulation potential of 71 strains. These strains were the closest relatives of six genera namely <i>Aurantiochytrium</i>, <i>Botryochytrium</i>, <i>Parietichytrium</i>, <i>Schizochytrium</i>, <i>Thraustochytrium</i>, and <i>Labyrinthula</i>. Docosahexaenoic acid (DHA) production of the top 15 strains ranged from 0.23 g/L to 1.14 g/L. Two labyrinthulid strains, GXBH-107 and GXBH-215, exhibited unprecedented high DHA production potential with content >10% of biomass. Among all strains, ZJWZ-7, identified as an <i>Aurantiochytrium</i> strain, exhibited the highest DHA production. Further optimization of culture conditions for strain ZJWZ-7 showed improved lipid production (1.66 g/L DHA and 1.68 g/L saturated fatty acids (SFAs)) with glycerol-malic-acid, peptone-yeast-extract, initial pH 7, 28 °C, and rotation rate 150 rpm. Besides, nitrogen source, initial pH, temperature, and rotation rate had significant effects on the cell biomass, DHA, and SFAs production. This study provides the identification and characterization of nearly six dozen thraustochytrids and labyrinthulids with high potential for lipid accumulation.https://www.mdpi.com/1660-3397/17/5/268lipid profilingthraustochytridslabyrinthulidsisolationphylogenybatch cultureoptimization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiuzhen Wang Huike Ye Yunxuan Xie Yaodong He Biswarup Sen Guangyi Wang |
spellingShingle |
Qiuzhen Wang Huike Ye Yunxuan Xie Yaodong He Biswarup Sen Guangyi Wang Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China Marine Drugs lipid profiling thraustochytrids labyrinthulids isolation phylogeny batch culture optimization |
author_facet |
Qiuzhen Wang Huike Ye Yunxuan Xie Yaodong He Biswarup Sen Guangyi Wang |
author_sort |
Qiuzhen Wang |
title |
Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China |
title_short |
Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China |
title_full |
Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China |
title_fullStr |
Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China |
title_full_unstemmed |
Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China |
title_sort |
culturable diversity and lipid production profile of labyrinthulomycete protists isolated from coastal mangrove habitats of china |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2019-05-01 |
description |
Labyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, their lipid profiles are poorly described because only a few large-scale isolation attempts have been made so far. Here, we isolated more than 200 strains from mangrove habitats of China and characterized the molecular phylogeny and lipid accumulation potential of 71 strains. These strains were the closest relatives of six genera namely <i>Aurantiochytrium</i>, <i>Botryochytrium</i>, <i>Parietichytrium</i>, <i>Schizochytrium</i>, <i>Thraustochytrium</i>, and <i>Labyrinthula</i>. Docosahexaenoic acid (DHA) production of the top 15 strains ranged from 0.23 g/L to 1.14 g/L. Two labyrinthulid strains, GXBH-107 and GXBH-215, exhibited unprecedented high DHA production potential with content >10% of biomass. Among all strains, ZJWZ-7, identified as an <i>Aurantiochytrium</i> strain, exhibited the highest DHA production. Further optimization of culture conditions for strain ZJWZ-7 showed improved lipid production (1.66 g/L DHA and 1.68 g/L saturated fatty acids (SFAs)) with glycerol-malic-acid, peptone-yeast-extract, initial pH 7, 28 °C, and rotation rate 150 rpm. Besides, nitrogen source, initial pH, temperature, and rotation rate had significant effects on the cell biomass, DHA, and SFAs production. This study provides the identification and characterization of nearly six dozen thraustochytrids and labyrinthulids with high potential for lipid accumulation. |
topic |
lipid profiling thraustochytrids labyrinthulids isolation phylogeny batch culture optimization |
url |
https://www.mdpi.com/1660-3397/17/5/268 |
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