Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia
ABSTRACT Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers. Materials and Methods: A total of eight N...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Veterinary World
2017-04-01
|
Series: | Veterinary World |
Subjects: | |
Online Access: | http://www.veterinaryworld.org/Vol.10/April-2017/11.pdf |
id |
doaj-e8e42d2892504cb7be22dc47e32f255c |
---|---|
record_format |
Article |
spelling |
doaj-e8e42d2892504cb7be22dc47e32f255c2021-08-02T09:45:08ZengVeterinary WorldVeterinary World0972-89882231-09162017-04-0110443844410.14202/vetworld.2017.438-444Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, IndonesiaDwi Desmiyeni Putri0Ekowati Handharyani1Retno Damajanti Soejoedono2Agus Setiyono3Ni Luh Putu Ika Mayasari4Okti Nadia Poetri5Study Program of Animal Biomedical Science, IPB Graduate School, Bogor Agricultural University, Bogor, Indonesia; Study Program of Animal Husbandry, Department of Animal Husbandry, State Polytechnic of Lampung, Lampung, Indonesia.Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.ABSTRACT Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers. Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains. Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R/G-F117 on NDV virulent strains and 112G-K/R-Q-G-R-L117 on NDV avirulent strain. Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.http://www.veterinaryworld.org/Vol.10/April-2017/11.pdfF geneM genemolecular pathotypingNewcastle disease virusvaccinated chickensvirulence. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dwi Desmiyeni Putri Ekowati Handharyani Retno Damajanti Soejoedono Agus Setiyono Ni Luh Putu Ika Mayasari Okti Nadia Poetri |
spellingShingle |
Dwi Desmiyeni Putri Ekowati Handharyani Retno Damajanti Soejoedono Agus Setiyono Ni Luh Putu Ika Mayasari Okti Nadia Poetri Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia Veterinary World F gene M gene molecular pathotyping Newcastle disease virus vaccinated chickens virulence. |
author_facet |
Dwi Desmiyeni Putri Ekowati Handharyani Retno Damajanti Soejoedono Agus Setiyono Ni Luh Putu Ika Mayasari Okti Nadia Poetri |
author_sort |
Dwi Desmiyeni Putri |
title |
Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia |
title_short |
Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia |
title_full |
Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia |
title_fullStr |
Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia |
title_full_unstemmed |
Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia |
title_sort |
pathotypic characterization of newcastle disease virus isolated from vaccinated chicken in west java, indonesia |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2017-04-01 |
description |
ABSTRACT
Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.
Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.
Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R/G-F117 on NDV virulent strains and 112G-K/R-Q-G-R-L117 on NDV avirulent strain.
Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry. |
topic |
F gene M gene molecular pathotyping Newcastle disease virus vaccinated chickens virulence. |
url |
http://www.veterinaryworld.org/Vol.10/April-2017/11.pdf |
work_keys_str_mv |
AT dwidesmiyeniputri pathotypiccharacterizationofnewcastlediseasevirusisolatedfromvaccinatedchickeninwestjavaindonesia AT ekowatihandharyani pathotypiccharacterizationofnewcastlediseasevirusisolatedfromvaccinatedchickeninwestjavaindonesia AT retnodamajantisoejoedono pathotypiccharacterizationofnewcastlediseasevirusisolatedfromvaccinatedchickeninwestjavaindonesia AT agussetiyono pathotypiccharacterizationofnewcastlediseasevirusisolatedfromvaccinatedchickeninwestjavaindonesia AT niluhputuikamayasari pathotypiccharacterizationofnewcastlediseasevirusisolatedfromvaccinatedchickeninwestjavaindonesia AT oktinadiapoetri pathotypiccharacterizationofnewcastlediseasevirusisolatedfromvaccinatedchickeninwestjavaindonesia |
_version_ |
1721234606312652800 |