Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions o...

Full description

Bibliographic Details
Main Authors: Xiao-fei LIU, Wei-min SUN, Ze-qin LI, Rui-xue BAI, Jing-xiao LI, Zi-han SHI, Hongwei GENG, Ying ZHENG, Jun ZHANG, Gen-fa ZHANG
Format: Article
Language:English
Published: Elsevier 2013-10-01
Series:Journal of Integrative Agriculture
Subjects:
drought
jojoba
promoter
ROS
ScMnSOD
stress tolerance
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311913604049
id doaj-e8f0f8c7f85e4b9fb68edcd6fab42ba5
record_format Article
spelling doaj-e8f0f8c7f85e4b9fb68edcd6fab42ba52021-06-07T06:48:50ZengElsevierJournal of Integrative Agriculture2095-31192013-10-01121017221730Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in ArabidopsisXiao-fei LIU0Wei-min SUN1Ze-qin LI2Rui-xue BAI3Jing-xiao LI4Zi-han SHI5Hongwei GENG6Ying ZHENG7Jun ZHANG8Gen-fa ZHANG9Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. China; College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. China; College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. China; College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. China; College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. China; College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. ChinaBeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. ChinaCOE For Neuroscience, Departments of Anesthesiology, Biomedical Sciences, Texas Tech University Health Sciences Center, Texas 79905, USABeijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R. China; College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. China; Correspondence ZHANG Gen-fa, Tel: +86-10-58809453, Fax: +86-10-58807720Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5′-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.http://www.sciencedirect.com/science/article/pii/S2095311913604049droughtjojobapromoterROSScMnSODstress tolerance
collection DOAJ
language English
format Article
sources DOAJ
author Xiao-fei LIU
Wei-min SUN
Ze-qin LI
Rui-xue BAI
Jing-xiao LI
Zi-han SHI
Hongwei GENG
Ying ZHENG
Jun ZHANG
Gen-fa ZHANG
spellingShingle Xiao-fei LIU
Wei-min SUN
Ze-qin LI
Rui-xue BAI
Jing-xiao LI
Zi-han SHI
Hongwei GENG
Ying ZHENG
Jun ZHANG
Gen-fa ZHANG
Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
Journal of Integrative Agriculture
drought
jojoba
promoter
ROS
ScMnSOD
stress tolerance
author_facet Xiao-fei LIU
Wei-min SUN
Ze-qin LI
Rui-xue BAI
Jing-xiao LI
Zi-han SHI
Hongwei GENG
Ying ZHENG
Jun ZHANG
Gen-fa ZHANG
author_sort Xiao-fei LIU
title Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
title_short Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
title_full Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
title_fullStr Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
title_full_unstemmed Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
title_sort over-expression of scmnsod, a sod gene derived from jojoba, improve drought tolerance in arabidopsis
publisher Elsevier
series Journal of Integrative Agriculture
issn 2095-3119
publishDate 2013-10-01
description Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5′-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.
topic drought
jojoba
promoter
ROS
ScMnSOD
stress tolerance
url http://www.sciencedirect.com/science/article/pii/S2095311913604049
work_keys_str_mv AT xiaofeiliu overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT weiminsun overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT zeqinli overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT ruixuebai overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT jingxiaoli overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT zihanshi overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT hongweigeng overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT yingzheng overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT junzhang overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
AT genfazhang overexpressionofscmnsodasodgenederivedfromjojobaimprovedroughttoleranceinarabidopsis
_version_ 1724163516614574080

Similar Items