Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women
Abstract Background High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological cancer among Tunisian women. Preventive strategies against CC are based on prophylactic vaccines that have not yet been implemented into the n...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-07-01
|
Series: | Infectious Agents and Cancer |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13027-021-00392-1 |
id |
doaj-e8f5e496ef3b4c6d96f0f41d3a6f1f87 |
---|---|
record_format |
Article |
spelling |
doaj-e8f5e496ef3b4c6d96f0f41d3a6f1f872021-07-18T11:14:08ZengBMCInfectious Agents and Cancer1750-93782021-07-011611710.1186/s13027-021-00392-1Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian womenRahima Bel Haj Rhouma0Monia Ardhaoui1Emna El Fehri2Asma Marzougui3Thalja Laassili4Ikram Guizani5Med Samir Boubaker6Emna Ennaifer7Department of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarDepartment of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarDepartment of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarDepartment of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarDepartment of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarLaboratory of Molecular Epidemiology and Infectious Diseases, Pasteur Institute of Tunis, University Tunis El ManarDepartment of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarDepartment of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El ManarAbstract Background High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological cancer among Tunisian women. Preventive strategies against CC are based on prophylactic vaccines that have not yet been implemented into the national vaccination program of Tunisia. Therefore, the present study aimed to investigate the HPV genotypes distribution in cervical neoplasia in Tunisian women in order to predict the impact of using current HPV vaccines on cancer prevention in Tunisia. Methods A total of 200 formalin-fixed paraffin embedded biopsies were collected in our study. DNA was extracted using Qiagen Mini prep kit. DNA quality was controlled by Beta Globin PCR. Only positive samples for Beta Globin test were used. HPV detection was performed by a nested PCR using PYGMY and GP5+/6+ primers. Genotyping was performed by Reverse Line hybridization using 31 probes. Results The mean age of participants was 38.97 years and 75% were over 30 years. Cervical neoplasia distribution according to age showed that CINII/CINIII was observed among women over 30 years old. All samples were positive for Beta Globin PCR. Overall HPV prevalence in cervical lesions was 83% (166/200). HPV was present in 65% of CINI, 82% of CINII/CINIII and 85% of CC. HR-HPV was statistically significantly associated with cervical intraepithelial neoplasia (p < 10–3). HR-HPV distribution according to lesion grade and cervical cancer showed that HPV16 and HPV18 were present in all lesions. For CINII/CINIII, HPV 35 (37.5%) was the most detected type, followed by HPV18 (33.3%) HPV 45 (28.5%) and HPV 16 (18.9%). HPV 45(57.5%), HPV 18 (53.3%) were the most detected in CC. HPV58, 59, 68 were only detected in CC and associated with HPV45, 18 and HPV16. HPV39, 31, 33, 52, 56 and HPV70 was associated only with CINI. Conclusions Our findings can give useful information for vaccine implementation by helping the health policymakers to choose the most appropriate vaccine type in Tunisia.https://doi.org/10.1186/s13027-021-00392-1Cervical neoplasiaCervical cancerHuman papillomavirusGenotype distribution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rahima Bel Haj Rhouma Monia Ardhaoui Emna El Fehri Asma Marzougui Thalja Laassili Ikram Guizani Med Samir Boubaker Emna Ennaifer |
spellingShingle |
Rahima Bel Haj Rhouma Monia Ardhaoui Emna El Fehri Asma Marzougui Thalja Laassili Ikram Guizani Med Samir Boubaker Emna Ennaifer Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women Infectious Agents and Cancer Cervical neoplasia Cervical cancer Human papillomavirus Genotype distribution |
author_facet |
Rahima Bel Haj Rhouma Monia Ardhaoui Emna El Fehri Asma Marzougui Thalja Laassili Ikram Guizani Med Samir Boubaker Emna Ennaifer |
author_sort |
Rahima Bel Haj Rhouma |
title |
Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women |
title_short |
Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women |
title_full |
Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women |
title_fullStr |
Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women |
title_full_unstemmed |
Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women |
title_sort |
distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in tunisian women |
publisher |
BMC |
series |
Infectious Agents and Cancer |
issn |
1750-9378 |
publishDate |
2021-07-01 |
description |
Abstract Background High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological cancer among Tunisian women. Preventive strategies against CC are based on prophylactic vaccines that have not yet been implemented into the national vaccination program of Tunisia. Therefore, the present study aimed to investigate the HPV genotypes distribution in cervical neoplasia in Tunisian women in order to predict the impact of using current HPV vaccines on cancer prevention in Tunisia. Methods A total of 200 formalin-fixed paraffin embedded biopsies were collected in our study. DNA was extracted using Qiagen Mini prep kit. DNA quality was controlled by Beta Globin PCR. Only positive samples for Beta Globin test were used. HPV detection was performed by a nested PCR using PYGMY and GP5+/6+ primers. Genotyping was performed by Reverse Line hybridization using 31 probes. Results The mean age of participants was 38.97 years and 75% were over 30 years. Cervical neoplasia distribution according to age showed that CINII/CINIII was observed among women over 30 years old. All samples were positive for Beta Globin PCR. Overall HPV prevalence in cervical lesions was 83% (166/200). HPV was present in 65% of CINI, 82% of CINII/CINIII and 85% of CC. HR-HPV was statistically significantly associated with cervical intraepithelial neoplasia (p < 10–3). HR-HPV distribution according to lesion grade and cervical cancer showed that HPV16 and HPV18 were present in all lesions. For CINII/CINIII, HPV 35 (37.5%) was the most detected type, followed by HPV18 (33.3%) HPV 45 (28.5%) and HPV 16 (18.9%). HPV 45(57.5%), HPV 18 (53.3%) were the most detected in CC. HPV58, 59, 68 were only detected in CC and associated with HPV45, 18 and HPV16. HPV39, 31, 33, 52, 56 and HPV70 was associated only with CINI. Conclusions Our findings can give useful information for vaccine implementation by helping the health policymakers to choose the most appropriate vaccine type in Tunisia. |
topic |
Cervical neoplasia Cervical cancer Human papillomavirus Genotype distribution |
url |
https://doi.org/10.1186/s13027-021-00392-1 |
work_keys_str_mv |
AT rahimabelhajrhouma distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT moniaardhaoui distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT emnaelfehri distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT asmamarzougui distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT thaljalaassili distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT ikramguizani distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT medsamirboubaker distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen AT emnaennaifer distributionofhumanpapillomavirusinprecancerousandcancerouscervicalneoplasiaintunisianwomen |
_version_ |
1721296398848098304 |