| Summary: | Halophytic plants can adapt to grow and thrive in highly saline conditions. <i>Suaeda</i> species are annual halophytes with high salt tolerance and are most suitable in the restoration of salinized or contaminated saline land and as food, forage, medicine, and bioenergy. In this study, we comprehensively analyzed the different metabolic responses of <i>Suaeda</i> species under salt and drought stress at ambient and elevated CO<sub>2</sub> conditions. Seedlings of <i>Suaeda</i> species were treated with 500 mM NaCl and 5% of polyethylene glycol under elevated CO<sub>2</sub> stress conditions for 24 h. Then, widely untargeted metabolites were detected by gas chromatography–mass spectrometry. Different metabolites involved in amino acid metabolism, glycolysis, photorespiration, and tricarboxylic acid cycle were quantitatively determined after stress treatments. A total of 61 primary metabolites were annotated. Different treatments increased the contents of certain metabolites, such as amino acids, sugars, and organic acids, as well as some antioxidants, such as quininic acid, kaempferol, and melatonin. These substances may be correlated with osmotic tolerance, increased antioxidant activity, and medical and nutritional value in the species. This study suggests that various metabolites differentially accumulated in C<sub>4</sub><i>Suaeda</i> species under varying stress conditions. Furthermore, this work provides new insights into the key secondary metabolite pathway involved in stress tolerance.
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