In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)
In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniqu...
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doaj-e91b0dcfbbb8468795f34f20a47180ec2020-11-25T00:20:50ZengHindawi LimitedBioMed Research International2314-61332314-61412019-01-01201910.1155/2019/32648463264846In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)Jahangir Khan Achakzai0Muhammad Anwar Panezai1Muhammad Ayub Kakar2Abdul Manan Kakar3Shahabuddin Kakar4Javed Khan5Nazima Yousaf Khan6Inayatullah Khilji7Ajab Khan Tareen8Institute of Biochemistry, University of Balochistan, Quetta 87300, PakistanInstitute of Biochemistry, University of Balochistan, Quetta 87300, PakistanInstitute of Biochemistry, University of Balochistan, Quetta 87300, PakistanInstitute of Biochemistry, University of Balochistan, Quetta 87300, PakistanDepartment of Zoology, University of Balochistan, Quetta 87300, PakistanDepartment of Microbiology, Quaid-i-Azam University, Islamabad 45320, PakistanInstitute of Biochemistry, University of Balochistan, Quetta 87300, PakistanInstitute of Biochemistry, University of Balochistan, Quetta 87300, PakistanInstitute of Biochemistry, University of Balochistan, Quetta 87300, PakistanIn this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.http://dx.doi.org/10.1155/2019/3264846 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jahangir Khan Achakzai Muhammad Anwar Panezai Muhammad Ayub Kakar Abdul Manan Kakar Shahabuddin Kakar Javed Khan Nazima Yousaf Khan Inayatullah Khilji Ajab Khan Tareen |
spellingShingle |
Jahangir Khan Achakzai Muhammad Anwar Panezai Muhammad Ayub Kakar Abdul Manan Kakar Shahabuddin Kakar Javed Khan Nazima Yousaf Khan Inayatullah Khilji Ajab Khan Tareen In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR) BioMed Research International |
author_facet |
Jahangir Khan Achakzai Muhammad Anwar Panezai Muhammad Ayub Kakar Abdul Manan Kakar Shahabuddin Kakar Javed Khan Nazima Yousaf Khan Inayatullah Khilji Ajab Khan Tareen |
author_sort |
Jahangir Khan Achakzai |
title |
In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR) |
title_short |
In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR) |
title_full |
In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR) |
title_fullStr |
In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR) |
title_full_unstemmed |
In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR) |
title_sort |
in vitro anticancer mcf-7, anti-inflammatory, and brine shrimp lethality assay (bsla) and gc-ms analysis of whole plant butanol fraction of rheum ribes (wbfrr) |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2019-01-01 |
description |
In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects. |
url |
http://dx.doi.org/10.1155/2019/3264846 |
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