Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection
The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2012-04-01
|
Series: | Journal of Pharmaceutical Analysis |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177911000670 |
id |
doaj-e92619010a174f8488d74c6c06806f15 |
---|---|
record_format |
Article |
spelling |
doaj-e92619010a174f8488d74c6c06806f152021-04-02T04:34:02ZengElsevierJournal of Pharmaceutical Analysis2095-17792012-04-0122143151Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detectionQinhua Chen0Yuan Li1Zilin Chen2Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, ChinaKey Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, ChinaCorresponding author. Tel.: +86 27 68759893; fax: +86 27 68759850.; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, ChinaThe qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins, benzofuran glycosides, flavonoids, and meroterpene were identified. The determination of four major constituents including bavachin, isobavachalcone, bavachinin, and bakuchiol was accomplished by HPLC with UV, MS, and electrochemical detection (ECD). These methods were evaluated for a number of validation characteristics (repeatability, LOD, calibration range, and recovery). ECD obtained a high sensitivity for analysis of the four components; MS provided a high selectivity and sensitivity for determination of bavachin, isobavachalcone, and bavachinin in negative-ion mode. After optimization of the methods, separation, identification. and quantification of the four components in Fructus Psoraleae were comprehensively tested by HPLC with UV, MS, and ECD. Keywords: HPLC-UV, HPLC-MS, HPLC-ECD, Fructus Psoraleae, Chinese medicinehttp://www.sciencedirect.com/science/article/pii/S2095177911000670 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qinhua Chen Yuan Li Zilin Chen |
spellingShingle |
Qinhua Chen Yuan Li Zilin Chen Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection Journal of Pharmaceutical Analysis |
author_facet |
Qinhua Chen Yuan Li Zilin Chen |
author_sort |
Qinhua Chen |
title |
Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection |
title_short |
Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection |
title_full |
Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection |
title_fullStr |
Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection |
title_full_unstemmed |
Separation, identification, and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV, ion trap mass spectrometry, and electrochemical detection |
title_sort |
separation, identification, and quantification of active constituents in fructus psoraleae by high-performance liquid chromatography with uv, ion trap mass spectrometry, and electrochemical detection |
publisher |
Elsevier |
series |
Journal of Pharmaceutical Analysis |
issn |
2095-1779 |
publishDate |
2012-04-01 |
description |
The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins, benzofuran glycosides, flavonoids, and meroterpene were identified. The determination of four major constituents including bavachin, isobavachalcone, bavachinin, and bakuchiol was accomplished by HPLC with UV, MS, and electrochemical detection (ECD). These methods were evaluated for a number of validation characteristics (repeatability, LOD, calibration range, and recovery). ECD obtained a high sensitivity for analysis of the four components; MS provided a high selectivity and sensitivity for determination of bavachin, isobavachalcone, and bavachinin in negative-ion mode. After optimization of the methods, separation, identification. and quantification of the four components in Fructus Psoraleae were comprehensively tested by HPLC with UV, MS, and ECD. Keywords: HPLC-UV, HPLC-MS, HPLC-ECD, Fructus Psoraleae, Chinese medicine |
url |
http://www.sciencedirect.com/science/article/pii/S2095177911000670 |
work_keys_str_mv |
AT qinhuachen separationidentificationandquantificationofactiveconstituentsinfructuspsoraleaebyhighperformanceliquidchromatographywithuviontrapmassspectrometryandelectrochemicaldetection AT yuanli separationidentificationandquantificationofactiveconstituentsinfructuspsoraleaebyhighperformanceliquidchromatographywithuviontrapmassspectrometryandelectrochemicaldetection AT zilinchen separationidentificationandquantificationofactiveconstituentsinfructuspsoraleaebyhighperformanceliquidchromatographywithuviontrapmassspectrometryandelectrochemicaldetection |
_version_ |
1724173310819827712 |