Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy
Endocrine and metabolic disorders are a common condition in Europe and worldwide, and, among these, thyroid dysfunction still remains a problem. The measurement of thyroid stimulating hormone (TSH) levels represents the first-line assay for the assessment of thyroid function. In the present study, w...
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doaj-e9525116c5d048a79c8d4d95ded3fcfb2021-09-25T23:59:01ZengMDPI AGDiagnostics2075-44182021-08-01111590159010.3390/diagnostics11091590Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern ItalyAlfredo Di Cerbo0Nazario Quagliano1Antonella Napolitano2Federica Pezzuto3Tommaso Iannitti4Alessandro Di Cerbo5Leonardo da Vinci Private Clinic, Via Nicola De Dominicis, 71121 Foggia, ItalyBiometron Laboratory, Viale Aldo Moro, 122/128, 71011 Apricena, ItalyBiometron Laboratory, Viale Aldo Moro, 122/128, 71011 Apricena, ItalyLeonardo da Vinci Private Clinic, Via Nicola De Dominicis, 71121 Foggia, ItalyIndependent Researcher, Southampton SO16 0BS, UKSchool of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, ItalyEndocrine and metabolic disorders are a common condition in Europe and worldwide, and, among these, thyroid dysfunction still remains a problem. The measurement of thyroid stimulating hormone (TSH) levels represents the first-line assay for the assessment of thyroid function. In the present study, we compared serum concentrations of TSH, measured using a commercially available point-of-care test (POCT) method (FastPack<sup>®</sup> IP) and an established “conventional” laboratory-based method (Beckmann Access 2) in a cohort of patients from Foggia in Southern Italy. A strong correlation (<i>r</i> = 0.994) was found between both methods and was also confirmed by receiver operating characteristic (ROC) curve analysis (0.82). The within-run coefficient of variation (CV) using FastPack<sup>®</sup> ranged from 4.03% and 8.57% at the TSH concentrations of 39.49 and 0.70 mIU/L, respectively. The between-run CV was 10.34% and 6.33% at the TSH concentrations of 0.87 and 26.55 mIU/L, respectively. The ratios of within- to between-assay CV were 0.83 and 1.06 at the TSH levels of 0.70 and 52.59 mIU/mL, respectively. In this study, we showed that serum TSH levels can be measured in a few minutes and at low-cost in terms of materials and equipment required. We observed that this approach is user-friendly, accurate, reproducible, and suitable for use in the clinic, while also meeting the criteria for effectiveness, impact, efficiency, and sustainability.https://www.mdpi.com/2075-4418/11/9/1590endocrine and metabolic disordersthyroid stimulating hormonepoint-of-care test |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alfredo Di Cerbo Nazario Quagliano Antonella Napolitano Federica Pezzuto Tommaso Iannitti Alessandro Di Cerbo |
spellingShingle |
Alfredo Di Cerbo Nazario Quagliano Antonella Napolitano Federica Pezzuto Tommaso Iannitti Alessandro Di Cerbo Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy Diagnostics endocrine and metabolic disorders thyroid stimulating hormone point-of-care test |
author_facet |
Alfredo Di Cerbo Nazario Quagliano Antonella Napolitano Federica Pezzuto Tommaso Iannitti Alessandro Di Cerbo |
author_sort |
Alfredo Di Cerbo |
title |
Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy |
title_short |
Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy |
title_full |
Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy |
title_fullStr |
Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy |
title_full_unstemmed |
Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy |
title_sort |
comparison between an emerging point-of-care tool for tsh evaluation and a centralized laboratory-based method in a cohort of patients from southern italy |
publisher |
MDPI AG |
series |
Diagnostics |
issn |
2075-4418 |
publishDate |
2021-08-01 |
description |
Endocrine and metabolic disorders are a common condition in Europe and worldwide, and, among these, thyroid dysfunction still remains a problem. The measurement of thyroid stimulating hormone (TSH) levels represents the first-line assay for the assessment of thyroid function. In the present study, we compared serum concentrations of TSH, measured using a commercially available point-of-care test (POCT) method (FastPack<sup>®</sup> IP) and an established “conventional” laboratory-based method (Beckmann Access 2) in a cohort of patients from Foggia in Southern Italy. A strong correlation (<i>r</i> = 0.994) was found between both methods and was also confirmed by receiver operating characteristic (ROC) curve analysis (0.82). The within-run coefficient of variation (CV) using FastPack<sup>®</sup> ranged from 4.03% and 8.57% at the TSH concentrations of 39.49 and 0.70 mIU/L, respectively. The between-run CV was 10.34% and 6.33% at the TSH concentrations of 0.87 and 26.55 mIU/L, respectively. The ratios of within- to between-assay CV were 0.83 and 1.06 at the TSH levels of 0.70 and 52.59 mIU/mL, respectively. In this study, we showed that serum TSH levels can be measured in a few minutes and at low-cost in terms of materials and equipment required. We observed that this approach is user-friendly, accurate, reproducible, and suitable for use in the clinic, while also meeting the criteria for effectiveness, impact, efficiency, and sustainability. |
topic |
endocrine and metabolic disorders thyroid stimulating hormone point-of-care test |
url |
https://www.mdpi.com/2075-4418/11/9/1590 |
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