Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo
Local anesthetics (LAs) are drugs that promote the reversible blocking of neural transmission by inhibiting the excitation conduction process in peripheral nerves. Tetracaine (TTC) is one of the most common topical anesthetics used in general practice and was applied to provide long-term anesthesia....
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2019-09-01
|
Series: | Biomedicine & Pharmacotherapy |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332219317664 |
id |
doaj-e95f6019a6624d128de2365d1b5feb26 |
---|---|
record_format |
Article |
spelling |
doaj-e95f6019a6624d128de2365d1b5feb262021-05-20T07:38:28ZengElsevierBiomedicine & Pharmacotherapy0753-33222019-09-01117Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivoXiaoli Liu0Qianqian Zhao1Department of Anesthesiology, Affiliated Hospital of Jining Medical University, Jining City, 272000, Shandong Province, PR ChinaCorresponding author at: Address: No. 89 Guhuai Road, Affiliated Hospital of Jining Medical University, Jining City, 272000, Shandong Province, PR China.; Department of Anesthesiology, Affiliated Hospital of Jining Medical University, Jining City, 272000, Shandong Province, PR ChinaLocal anesthetics (LAs) are drugs that promote the reversible blocking of neural transmission by inhibiting the excitation conduction process in peripheral nerves. Tetracaine (TTC) is one of the most common topical anesthetics used in general practice and was applied to provide long-term anesthesia. In this research, poly(L-lactide) nanoparticles (PLA NPs), solid lipid nanoparticles (SLNs), and nanostructured lipid carriers (NLCs) were utilized to construct TTC loaded nanosystems. The mean sizes, drug loading efficiency, cytotoxicity, skin permeation ability, and anesthetic analgesic effect were evaluated and compared in vitro and in vivo. The average particle sizes of blank PLA NPs, SLNs, and NLCs were 93.2, 100.9 and 110.4 nm, respectively. At all the concentrations, PLA NPs, SLNs, and NLCs showed a moderate effect on cell viability. TTC NLCs exhibited the most prominent in vivo efficiency in improving the skin permeation, analgesic time and pain control intensity. Other experiments proved that TTC PLA NPs showed advantages in serum stability and TTC SLNs illustrated the best in vitro permeation efficiency. These three kinds of nano-systems had their own superiority in some respects. Conclusion could be made that in this study, TTC NLCs is the promising system for the long-term anesthesia.http://www.sciencedirect.com/science/article/pii/S0753332219317664Local anestheticsNanotechnologyPermeation abilityAnesthetic analgesic effect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoli Liu Qianqian Zhao |
spellingShingle |
Xiaoli Liu Qianqian Zhao Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo Biomedicine & Pharmacotherapy Local anesthetics Nanotechnology Permeation ability Anesthetic analgesic effect |
author_facet |
Xiaoli Liu Qianqian Zhao |
author_sort |
Xiaoli Liu |
title |
Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo |
title_short |
Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo |
title_full |
Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo |
title_fullStr |
Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo |
title_full_unstemmed |
Long-term anesthetic analgesic effects: Comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo |
title_sort |
long-term anesthetic analgesic effects: comparison of tetracaine loaded polymeric nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers in vitro and in vivo |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2019-09-01 |
description |
Local anesthetics (LAs) are drugs that promote the reversible blocking of neural transmission by inhibiting the excitation conduction process in peripheral nerves. Tetracaine (TTC) is one of the most common topical anesthetics used in general practice and was applied to provide long-term anesthesia. In this research, poly(L-lactide) nanoparticles (PLA NPs), solid lipid nanoparticles (SLNs), and nanostructured lipid carriers (NLCs) were utilized to construct TTC loaded nanosystems. The mean sizes, drug loading efficiency, cytotoxicity, skin permeation ability, and anesthetic analgesic effect were evaluated and compared in vitro and in vivo. The average particle sizes of blank PLA NPs, SLNs, and NLCs were 93.2, 100.9 and 110.4 nm, respectively. At all the concentrations, PLA NPs, SLNs, and NLCs showed a moderate effect on cell viability. TTC NLCs exhibited the most prominent in vivo efficiency in improving the skin permeation, analgesic time and pain control intensity. Other experiments proved that TTC PLA NPs showed advantages in serum stability and TTC SLNs illustrated the best in vitro permeation efficiency. These three kinds of nano-systems had their own superiority in some respects. Conclusion could be made that in this study, TTC NLCs is the promising system for the long-term anesthesia. |
topic |
Local anesthetics Nanotechnology Permeation ability Anesthetic analgesic effect |
url |
http://www.sciencedirect.com/science/article/pii/S0753332219317664 |
work_keys_str_mv |
AT xiaoliliu longtermanestheticanalgesiceffectscomparisonoftetracaineloadedpolymericnanoparticlessolidlipidnanoparticlesandnanostructuredlipidcarriersinvitroandinvivo AT qianqianzhao longtermanestheticanalgesiceffectscomparisonoftetracaineloadedpolymericnanoparticlessolidlipidnanoparticlesandnanostructuredlipidcarriersinvitroandinvivo |
_version_ |
1721435390814978048 |