Proteomic characterization of the <it>Rph15</it> barley resistance gene-mediated defence responses to leaf rust

• Main Authors: Bernardo Letizia, Prinsi Bhakti, Negri Alfredo Simone, Cattivelli Luigi, Espen Luca, Valè Giampiero
• Format: Article
• Language: English
• Published: BMC 2012-11-01
• Series: BMC Genomics
• Subjects: Barley; Leaf rust; Resistance gene; Rph15; Proteomics; Near isogenic lines
• Online Access: http://www.biomedcentral.com/1471-2164/13/642

<p>Abstract</p> <p>Background</p> <p>Leaf rust, caused by the biotrophic fungal pathogen <it>Puccinia hordei</it>, is one of the most important foliar disease of barley (<it>Hordeum vulgare</it>) and represents a serious threat in many production regions of the world. The leaf rust resistance gene <it>Rph15</it> is of outstanding interest for resistance breeding because it confers resistance to over 350 <it>Puccinia hordei</it> isolates collected from around the world. Molecular and biochemical mechanisms responsible for the <it>Rph15</it> effectiveness are currently not investigated. The aim of the present work was to study the <it>Rph15</it>-based defence responses using a proteomic approach.</p> <p>Results</p> <p>Protein pattern changes in response to the leaf rust pathogen infection were investigated in two barley near isogenic lines (NILs), Bowman (leaf rust susceptible) and Bowman-<it>Rph15</it> (leaf rust resistant), differing for the introgression of the leaf rust resistance gene <it>Rph15</it>. Two infection time points, 24 hours and four days post inoculation (dpi), were analysed. No statistically significant differences were identified at the early time point, while at 4 dpi eighteen protein spots were significantly up or down regulated with a fold-change equal or higher than two in response to pathogen infection. Almost all the pathogen-responsive proteins were identified in the Bowman-<it>Rph15</it> resistant NIL. Protein spots were characterized by LC-MS/MS analysis and found to be involved in photosynthesis and energy metabolism, carbohydrate metabolism, protein degradation and defence. Proteomic data were complemented by transcriptional analysis of the respective genes. The identified proteins can be related to modulation of the photosynthetic apparatus components, re-direction of the metabolism to sustain defence responses and deployment of defence proteins.</p> <p>Conclusions</p> <p>The identification of leaf rust infection-modulated defence responses restricted to the resistant NIL support the hypothesis that basal defence responses of Bowman, but not the <it>Rph15</it> resistance gene-based ones, are suppressed or delayed by pathogen effectors to levels below the detection power of the adopted proteomic approach. Additionally, <it>Rph15</it>-mediated resistance processes identified mainly resides on a modulation of primary metabolism, affecting photosyntesis and carbohydrate pool.</p>