High-resolution and specific detection of bacteria on complex surfaces using nanoparticle probes and electron microscopy.
The study of the interaction of bacteria with surfaces requires the detection of specific bacterial groups with high spatial resolution. Here, we describe a method to rapidly and efficiently add nanogold particles to oligonucleotide probes, which target bacterial ribosomal RNA. These nanogold-labele...
Main Authors: | Jun Ye, Shaun Nielsen, Stephen Joseph, Torsten Thomas |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4446341?pdf=render |
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