Rationales and Approaches for Studying Metabolism in Eukaryotic Microalgae

Rationales and Approaches for Studying Metabolism in Eukaryotic Microalgae

The generation of efficient production strains is essential for the use of eukaryotic microalgae for biofuel production. Systems biology approaches including metabolite profiling on promising microalgal strains, will provide a better understanding of their metabolic networks, which is crucial for m...

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Bibliographic Details
Main Authors: Daniel Veyel, Alexander Erban, Ines Fehrle, Joachim Kopka, Michael Schroda
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:Metabolites
Subjects:
Chlamydomonas reinhardtii
metabolic engineering
biofuels
metabolite profiling
GC-MS
systems biology
Online Access:http://www.mdpi.com/2218-1989/4/2/184
Description
Summary:The generation of efficient production strains is essential for the use of eukaryotic microalgae for biofuel production. Systems biology approaches including metabolite profiling on promising microalgal strains, will provide a better understanding of their metabolic networks, which is crucial for metabolic engineering efforts. Chlamydomonas reinhardtii represents a suited model system for this purpose. We give an overview to genetically amenable microalgal strains with the potential for biofuel production and provide a critical review of currently used protocols for metabolite profiling on Chlamydomonas. We provide our own experimental data to underpin the validity of the conclusions drawn.
ISSN:2218-1989

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