Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L.
Genetic resistance to Potato Leafroll Virus (PLRV) is polygenic, which hinders the obtainment of resistant cultivars. However, works carried out at the International Potato Center have identified an andigena accession, LOP-868, with high resistance level and low accumulation of PLRV due to the gene...
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doaj-e99ac58c948a45449bb17896b2eefd1c2020-11-24T21:25:09ZengBrazilian Society of Plant BreedingCrop Breeding and Applied Biotechnology1518-78531984-70332017-06-0117323624310.1590/1984-70332017v17n3a37Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L.Otávio Luiz Gomes Carneiro0Silvia Regina Rodrigues de Paula Ribeiro1Carolina Mariane Moreira2Marcio Lisboa Guedes3Danilo Hottis Lyra4César Augusto Brasil Pereira Pinto5Universidade de São Paulo (USP), Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Departamento de Genética, Avenida Pádua Dias, 11, 13.418-900, Piracicaba, SP, BrazilUniversidade Federal de Lavras (UFLA), Campus UFLA, Departamento de Biologia, CP 3037, 37.200-000, Lavras, MG, BrazilInstituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais, Campus Poços de Caldas, Avenida Dirce Pereira Rosa, 300, 37.713-100, Poços de Caldas, MG, BrazilUniversidade Federal de Lavras (UFLA), Campus UFLA, Departamento de Biologia, CP 3037, 37.200-000, Lavras, MG, BrazilUniversidade de São Paulo (USP), Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Departamento de Genética, Avenida Pádua Dias, 11, 13.418-900, Piracicaba, SP, BrazilUniversidade Federal de Lavras (UFLA), Campus UFLA, Departamento de Biologia, CP 3037, 37.200-000, Lavras, MG, BrazilGenetic resistance to Potato Leafroll Virus (PLRV) is polygenic, which hinders the obtainment of resistant cultivars. However, works carried out at the International Potato Center have identified an andigena accession, LOP-868, with high resistance level and low accumulation of PLRV due to the gene of major effect Rladg. We verify the transfer of the Rladg allele to clones of the cross between LOP-868 and UFLA clones, by using the SCAR RGASC850 molecular marker; to evaluate the reaction of these clones to PLRV by inoculating the virus using aphids; and to analyze their agronomic performance of clones. Among the clones inoculated with viruliferous aphids, 49.3% were negative to the serological test, indicating possible resistance. Clones containing the Rladg allele were identified by the RGASC850 molecular marker, which demonstrates the possibility of transferring the Rladg allele of resistance to PLRV from LOP-868 to Solanum tuberosum. Some clones that presented the Rladg allele are also promising for agronomic performance. http://www.sbmp.org.br/cbab/siscbab/uploads/bb7f427b-7f68-c6c5.pdfPotatodisease resistanceviral disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Otávio Luiz Gomes Carneiro Silvia Regina Rodrigues de Paula Ribeiro Carolina Mariane Moreira Marcio Lisboa Guedes Danilo Hottis Lyra César Augusto Brasil Pereira Pinto |
spellingShingle |
Otávio Luiz Gomes Carneiro Silvia Regina Rodrigues de Paula Ribeiro Carolina Mariane Moreira Marcio Lisboa Guedes Danilo Hottis Lyra César Augusto Brasil Pereira Pinto Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L. Crop Breeding and Applied Biotechnology Potato disease resistance viral disease |
author_facet |
Otávio Luiz Gomes Carneiro Silvia Regina Rodrigues de Paula Ribeiro Carolina Mariane Moreira Marcio Lisboa Guedes Danilo Hottis Lyra César Augusto Brasil Pereira Pinto |
author_sort |
Otávio Luiz Gomes Carneiro |
title |
Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L. |
title_short |
Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L. |
title_full |
Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L. |
title_fullStr |
Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L. |
title_full_unstemmed |
Introgression of the Rladg allele of resistance to potato leafroll virus in Solanum tuberosum L. |
title_sort |
introgression of the rladg allele of resistance to potato leafroll virus in solanum tuberosum l. |
publisher |
Brazilian Society of Plant Breeding |
series |
Crop Breeding and Applied Biotechnology |
issn |
1518-7853 1984-7033 |
publishDate |
2017-06-01 |
description |
Genetic resistance to Potato Leafroll Virus (PLRV) is polygenic, which hinders the obtainment of resistant cultivars. However, works carried out at the International Potato Center have identified an andigena accession, LOP-868, with high resistance level and low accumulation of PLRV due to the gene of major effect Rladg. We verify the transfer of the Rladg allele to clones of the cross between LOP-868 and UFLA clones, by using the SCAR RGASC850 molecular marker; to evaluate the reaction of these clones to PLRV by inoculating the virus using aphids; and to analyze their agronomic performance of clones. Among the clones inoculated with viruliferous aphids, 49.3% were negative to
the serological test, indicating possible resistance. Clones containing the Rladg allele were identified by the RGASC850 molecular marker, which demonstrates the possibility of transferring the Rladg allele of resistance to PLRV from LOP-868
to Solanum tuberosum. Some clones that presented the Rladg allele are also promising for agronomic performance.
|
topic |
Potato disease resistance viral disease |
url |
http://www.sbmp.org.br/cbab/siscbab/uploads/bb7f427b-7f68-c6c5.pdf |
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