Analysis of the nucleotide sequence of the guinea pig cytomegalovirus (GPCMV) genome

• Main Authors: Cui Xiaohong, Date Shailesh V, Choi K, McGregor Alistair, Schleiss Mark R, McVoy Michael A
• Format: Article
• Language: English
• Published: BMC 2008-11-01
• Series: Virology Journal
• Online Access: http://www.virologyj.com/content/5/1/139

<p>Abstract</p> <p>In this report we describe the genomic sequence of guinea pig cytomegalovirus (GPCMV) assembled from a tissue culture-derived bacterial artificial chromosome clone, plasmid clones of viral restriction fragments, and direct PCR sequencing of viral DNA. The GPCMV genome is 232,678 bp, excluding the terminal repeats, and has a GC content of 55%. A total of 105 open reading frames (ORFs) of > 100 amino acids with sequence and/or positional homology to other CMV ORFs were annotated. Positional and sequence homologs of human cytomegalovirus open reading frames <it>UL23 </it>through <it>UL122 </it>were identified. Homology with other cytomegaloviruses was most prominent in the central ~60% of the genome, with divergence of sequence and lack of conserved homologs at the respective genomic termini. Of interest, the GPCMV genome was found in many cases to bear stronger phylogenetic similarity to primate CMVs than to rodent CMVs. The sequence of GPCMV should facilitate vaccine and pathogenesis studies in this model of congenital CMV infection.</p>