The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells
Three-dimensional cell culturing to capture a life-like experimental environment has become a versatile tool for basic and clinical research. Mucosal and skin tissues can be grown as “organoids” in a petri dish and serve a wide variety of research questions. Here, we report our experience with human...
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doaj-e9ade32b66424784904810c9329e15de2020-12-02T00:02:21ZengMDPI AGViruses1999-49152020-12-01121375137510.3390/v12121375The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans CellsRobert Jackson0Jordan D. Lukacs1Ingeborg Zehbe2Biotechnology Program, Lakehead University, Thunder Bay, ON P7B 5E1, CanadaDepartment of Biology, Lakehead University, Thunder Bay, ON P7B 5E1, CanadaProbe Development and Biomarker Exploration, Thunder Bay Regional Health Research Institute, Thunder Bay, ON P7B 6V4, CanadaThree-dimensional cell culturing to capture a life-like experimental environment has become a versatile tool for basic and clinical research. Mucosal and skin tissues can be grown as “organoids” in a petri dish and serve a wide variety of research questions. Here, we report our experience with human cervical organoids which could also include an immune component, e.g., Langerhans cells. We employ commercially available human cervical keratinocytes and fibroblasts as well as a myeloid cell line matured and purified into langerin-positive Langerhans cells. These are then seeded on a layer of keratinocytes with underlying dermal equivalent. Using about 10-fold more than the reported number in healthy cervical tissue (1–3%), we obtain differentiated cervical epithelium after 14 days with ~1% being Langerhans cells. We provide a detailed protocol for interested researchers to apply the described “aseptic” organoid model for all sorts of investigations—with or without Langerhans cells.https://www.mdpi.com/1999-4915/12/12/1375cervicalkeratinocytesorganoidsMUTZ cell lineLangerhans cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Robert Jackson Jordan D. Lukacs Ingeborg Zehbe |
spellingShingle |
Robert Jackson Jordan D. Lukacs Ingeborg Zehbe The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells Viruses cervical keratinocytes organoids MUTZ cell line Langerhans cells |
author_facet |
Robert Jackson Jordan D. Lukacs Ingeborg Zehbe |
author_sort |
Robert Jackson |
title |
The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells |
title_short |
The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells |
title_full |
The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells |
title_fullStr |
The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells |
title_full_unstemmed |
The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells |
title_sort |
potentials and pitfalls of a human cervical organoid model including langerhans cells |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2020-12-01 |
description |
Three-dimensional cell culturing to capture a life-like experimental environment has become a versatile tool for basic and clinical research. Mucosal and skin tissues can be grown as “organoids” in a petri dish and serve a wide variety of research questions. Here, we report our experience with human cervical organoids which could also include an immune component, e.g., Langerhans cells. We employ commercially available human cervical keratinocytes and fibroblasts as well as a myeloid cell line matured and purified into langerin-positive Langerhans cells. These are then seeded on a layer of keratinocytes with underlying dermal equivalent. Using about 10-fold more than the reported number in healthy cervical tissue (1–3%), we obtain differentiated cervical epithelium after 14 days with ~1% being Langerhans cells. We provide a detailed protocol for interested researchers to apply the described “aseptic” organoid model for all sorts of investigations—with or without Langerhans cells. |
topic |
cervical keratinocytes organoids MUTZ cell line Langerhans cells |
url |
https://www.mdpi.com/1999-4915/12/12/1375 |
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