Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome
This study represents the first swine transcriptome hive plots created from gene set enrichment analysis (GSEA) data and provides a novel insight into the global transcriptome changes occurring in tracheobronchial lymph nodes (TBLN) and spanning the swine genome. RNA isolated from draining TBLN from...
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Online Access: | https://doi.org/10.4137/BBI.S30522 |
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doaj-e9adf628fb1847e6a33a91e9fa60ee212020-11-25T03:56:49ZengSAGE PublishingBioinformatics and Biology Insights1177-93222015-01-019s210.4137/BBI.S30522Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node TranscriptomeLaura C. Miller0Darrell O. Bayles1Eraldo L. Zanella2Kelly M. Lager3Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA, USA.Infectious Bacterial Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA, USA.College of Agriculture and Veterinary Medicine, University of Passo Fundo, RS, Brazil.Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA, USA.This study represents the first swine transcriptome hive plots created from gene set enrichment analysis (GSEA) data and provides a novel insight into the global transcriptome changes occurring in tracheobronchial lymph nodes (TBLN) and spanning the swine genome. RNA isolated from draining TBLN from 5-week-old pigs, either clinically infected with a feral isolate of Pseudorabies virus or uninfected, was interrogated using Illumina Digital Gene Expression Tag Profiling. More than 100 million tag sequences were observed, representing 4,064,189 unique 21-base sequences collected from TBLN at time points 1, 3, 6, and 14 days post-inoculation (dpi). Multidimensional statistical tests were applied to determine the significant changes in tag abundance, and then the tags were annotated. Hive plots were created to visualize the differential expression within the swine transcriptome defined by the Broad Institute's GSEA reference datasets between infected and uninfected animals, allowing us to directly compare different conditions.https://doi.org/10.4137/BBI.S30522 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laura C. Miller Darrell O. Bayles Eraldo L. Zanella Kelly M. Lager |
spellingShingle |
Laura C. Miller Darrell O. Bayles Eraldo L. Zanella Kelly M. Lager Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome Bioinformatics and Biology Insights |
author_facet |
Laura C. Miller Darrell O. Bayles Eraldo L. Zanella Kelly M. Lager |
author_sort |
Laura C. Miller |
title |
Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome |
title_short |
Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome |
title_full |
Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome |
title_fullStr |
Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome |
title_full_unstemmed |
Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome |
title_sort |
effects of pseudorabies virus infection on the tracheobronchial lymph node transcriptome |
publisher |
SAGE Publishing |
series |
Bioinformatics and Biology Insights |
issn |
1177-9322 |
publishDate |
2015-01-01 |
description |
This study represents the first swine transcriptome hive plots created from gene set enrichment analysis (GSEA) data and provides a novel insight into the global transcriptome changes occurring in tracheobronchial lymph nodes (TBLN) and spanning the swine genome. RNA isolated from draining TBLN from 5-week-old pigs, either clinically infected with a feral isolate of Pseudorabies virus or uninfected, was interrogated using Illumina Digital Gene Expression Tag Profiling. More than 100 million tag sequences were observed, representing 4,064,189 unique 21-base sequences collected from TBLN at time points 1, 3, 6, and 14 days post-inoculation (dpi). Multidimensional statistical tests were applied to determine the significant changes in tag abundance, and then the tags were annotated. Hive plots were created to visualize the differential expression within the swine transcriptome defined by the Broad Institute's GSEA reference datasets between infected and uninfected animals, allowing us to directly compare different conditions. |
url |
https://doi.org/10.4137/BBI.S30522 |
work_keys_str_mv |
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