Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole
Toxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of th...
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doaj-e9b34a7a69ed44948c90de459aa1d59d2020-11-24T21:26:39ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512011-01-01201110.1155/2011/473983473983Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous VacuoleS. Muñiz-Hernández0M. González del Carmen1M. Mondragón2C. Mercier3M. F. Cesbron4S. L. Mondragón-González5S. González6R. Mondragón7Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Avenue IPN No 2508, San Pedro Zacatenco, Gustavo A. Madero 07360 Mexico City, MexicoDepartamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Avenue IPN No 2508, San Pedro Zacatenco, Gustavo A. Madero 07360 Mexico City, MexicoDepartamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Avenue IPN No 2508, San Pedro Zacatenco, Gustavo A. Madero 07360 Mexico City, MexicoFacultad de Medicina, Universidad Veracruzana, Avenue Hidalgo, Esq. Carrillo Puerto s/n, Col. Centro, 94740, VER, MexicoLaboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042 Grenoble Cedex 9, FranceUnidad Profesional Interdisciplinaria en Ingeniería y Tecnologías Avanzadas, IPN, Avenue IPN 2580, Gustavo A. Madero 7340 Mexico City, MexicoUnidad de Microscopia Electrónica, CINVESTAV-IPN, MexicoDepartamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Avenue IPN No 2508, San Pedro Zacatenco, Gustavo A. Madero 07360 Mexico City, MexicoToxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during proliferation of tachyzoites probably through the secretion of components and a pinching event of the membrane at the posterior end. It contributes in the intravacuolar parasite organization by the membrane connection between the tachyzoites posterior end and the residual body membrane to give place to the rosette conformation. Radial distribution of parasites in rosettes favors an efficient exteriorization. Absence of the network and presence of atypical residual bodies in a ΔGRA2-HXGPRT knock-out mutant affected the intravacuolar organization of tachyzoites and their exteriorization.http://dx.doi.org/10.1155/2011/473983 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. Muñiz-Hernández M. González del Carmen M. Mondragón C. Mercier M. F. Cesbron S. L. Mondragón-González S. González R. Mondragón |
spellingShingle |
S. Muñiz-Hernández M. González del Carmen M. Mondragón C. Mercier M. F. Cesbron S. L. Mondragón-González S. González R. Mondragón Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole Journal of Biomedicine and Biotechnology |
author_facet |
S. Muñiz-Hernández M. González del Carmen M. Mondragón C. Mercier M. F. Cesbron S. L. Mondragón-González S. González R. Mondragón |
author_sort |
S. Muñiz-Hernández |
title |
Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole |
title_short |
Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole |
title_full |
Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole |
title_fullStr |
Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole |
title_full_unstemmed |
Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole |
title_sort |
contribution of the residual body in the spatial organization of toxoplasma gondii tachyzoites within the parasitophorous vacuole |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2011-01-01 |
description |
Toxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during proliferation of tachyzoites probably through the secretion of components and a pinching event of the membrane at the posterior end. It contributes in the intravacuolar parasite organization by the membrane connection between the tachyzoites posterior end and the residual body membrane to give place to the rosette conformation. Radial distribution of parasites in rosettes favors an efficient exteriorization. Absence of the network and presence of atypical residual bodies in a ΔGRA2-HXGPRT knock-out mutant affected the intravacuolar organization of tachyzoites and their exteriorization. |
url |
http://dx.doi.org/10.1155/2011/473983 |
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