Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients
Summary: T cell immunity toward SARS-CoV-2 spike (S-), membrane (M-), and nucleocapsid (N-) proteins may define COVID-19 severity. Therefore, we compare the SARS-CoV-2-reactive T cell responses in moderate, severe, and critical COVID-19 patients and unexposed donors. Overlapping peptide pools of all...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Cell Reports Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266637912030118X |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Constantin J. Thieme Moritz Anft Krystallenia Paniskaki Arturo Blazquez-Navarro Adrian Doevelaar Felix S. Seibert Bodo Hoelzer Margarethe Justine Konik Marc Moritz Berger Thorsten Brenner Clemens Tempfer Carsten Watzl Toni L. Meister Stephanie Pfaender Eike Steinmann Sebastian Dolff Ulf Dittmer Timm H. Westhoff Oliver Witzke Ulrik Stervbo Toralf Roch Nina Babel |
| spellingShingle |
Constantin J. Thieme Moritz Anft Krystallenia Paniskaki Arturo Blazquez-Navarro Adrian Doevelaar Felix S. Seibert Bodo Hoelzer Margarethe Justine Konik Marc Moritz Berger Thorsten Brenner Clemens Tempfer Carsten Watzl Toni L. Meister Stephanie Pfaender Eike Steinmann Sebastian Dolff Ulf Dittmer Timm H. Westhoff Oliver Witzke Ulrik Stervbo Toralf Roch Nina Babel Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients Cell Reports Medicine COVID-19 SARS-CoV-2 immunity spike nucleocapsid S/M/N protein-reactive T cells |
| author_facet |
Constantin J. Thieme Moritz Anft Krystallenia Paniskaki Arturo Blazquez-Navarro Adrian Doevelaar Felix S. Seibert Bodo Hoelzer Margarethe Justine Konik Marc Moritz Berger Thorsten Brenner Clemens Tempfer Carsten Watzl Toni L. Meister Stephanie Pfaender Eike Steinmann Sebastian Dolff Ulf Dittmer Timm H. Westhoff Oliver Witzke Ulrik Stervbo Toralf Roch Nina Babel |
| author_sort |
Constantin J. Thieme |
| title |
Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients |
| title_short |
Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients |
| title_full |
Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients |
| title_fullStr |
Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients |
| title_full_unstemmed |
Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients |
| title_sort |
robust t cell response toward spike, membrane, and nucleocapsid sars-cov-2 proteins is not associated with recovery in critical covid-19 patients |
| publisher |
Elsevier |
| series |
Cell Reports Medicine |
| issn |
2666-3791 |
| publishDate |
2020-09-01 |
| description |
Summary: T cell immunity toward SARS-CoV-2 spike (S-), membrane (M-), and nucleocapsid (N-) proteins may define COVID-19 severity. Therefore, we compare the SARS-CoV-2-reactive T cell responses in moderate, severe, and critical COVID-19 patients and unexposed donors. Overlapping peptide pools of all three proteins induce SARS-CoV-2-reactive T cell response with dominance of CD4+ over CD8+ T cells and demonstrate interindividual immunity against the three proteins. M-protein induces the highest frequencies of CD4+ T cells, suggesting its relevance for diagnosis and vaccination. The T cell response of critical COVID-19 patients is robust and comparable or even superior to non-critical patients. Virus clearance and COVID-19 survival are not associated with either SARS-CoV-2 T cell kinetics or magnitude of T cell responses, respectively. Thus, our data do not support the hypothesis of insufficient SARS-CoV-2-reactive immunity in critical COVID-19. Conversely, it indicates that activation of differentiated memory effector T cells could cause hyperreactivity and immunopathogenesis in critical patients. |
| topic |
COVID-19 SARS-CoV-2 immunity spike nucleocapsid S/M/N protein-reactive T cells |
| url |
http://www.sciencedirect.com/science/article/pii/S266637912030118X |
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doaj-e9bfff21d5a24fd6baf7d0d9d2173a5a2020-11-25T04:00:53ZengElsevierCell Reports Medicine2666-37912020-09-0116100092Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 PatientsConstantin J. Thieme0Moritz Anft1Krystallenia Paniskaki2Arturo Blazquez-Navarro3Adrian Doevelaar4Felix S. Seibert5Bodo Hoelzer6Margarethe Justine Konik7Marc Moritz Berger8Thorsten Brenner9Clemens Tempfer10Carsten Watzl11Toni L. Meister12Stephanie Pfaender13Eike Steinmann14Sebastian Dolff15Ulf Dittmer16Timm H. Westhoff17Oliver Witzke18Ulrik Stervbo19Toralf Roch20Nina Babel21Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, BIH Center for Regenerative Therapies, Berlin, Berlin, Germany; Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Berlin, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyCharité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, BIH Center for Regenerative Therapies, Berlin, Berlin, Germany; Ruhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyUniversity Duisburg-Essen, University Hospital Essen, Department of Infectious Diseases, West-German Centre for Infectious Diseases, Essen, North Rhine-Westphalia, GermanyUniversity Duisburg-Essen, University Hospital Essen, Department of Anesthesiology and Intensive Care Medicine, Essen, North Rhine-Westphalia, GermanyUniversity Duisburg-Essen, University Hospital Essen, Department of Anesthesiology and Intensive Care Medicine, Essen, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Department of Gynecology and Obstetrics, Herne, North Rhine-Westphalia, GermanyLeibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund (IfADo), Department of Immunology Dortmund, North Rhine-Westphalia, GermanyRuhr-University Bochum, Department of Molecular and Medical Virology, Bochum, North Rhine-Westphalia, GermanyRuhr-University Bochum, Department of Molecular and Medical Virology, Bochum, North Rhine-Westphalia, GermanyRuhr-University Bochum, Department of Molecular and Medical Virology, Bochum, North Rhine-Westphalia, GermanyUniversity Duisburg-Essen, University Hospital Essen, Department of Infectious Diseases, West-German Centre for Infectious Diseases, Essen, North Rhine-Westphalia, GermanyUniversity Duisburg-Essen, University Hospital Essen, Institute for Virology, Essen, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyUniversity Duisburg-Essen, University Hospital Essen, Department of Infectious Diseases, West-German Centre for Infectious Diseases, Essen, North Rhine-Westphalia, GermanyRuhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyCharité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, BIH Center for Regenerative Therapies, Berlin, Berlin, Germany; Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Berlin, Germany; Ruhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, GermanyCharité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, BIH Center for Regenerative Therapies, Berlin, Berlin, Germany; Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Berlin, Germany; Ruhr-University Bochum, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Center for Translational Medicine and Immune Diagnostics Laboratory, Medical Department I, Herne, North Rhine-Westphalia, Germany; Corresponding authorSummary: T cell immunity toward SARS-CoV-2 spike (S-), membrane (M-), and nucleocapsid (N-) proteins may define COVID-19 severity. Therefore, we compare the SARS-CoV-2-reactive T cell responses in moderate, severe, and critical COVID-19 patients and unexposed donors. Overlapping peptide pools of all three proteins induce SARS-CoV-2-reactive T cell response with dominance of CD4+ over CD8+ T cells and demonstrate interindividual immunity against the three proteins. M-protein induces the highest frequencies of CD4+ T cells, suggesting its relevance for diagnosis and vaccination. The T cell response of critical COVID-19 patients is robust and comparable or even superior to non-critical patients. Virus clearance and COVID-19 survival are not associated with either SARS-CoV-2 T cell kinetics or magnitude of T cell responses, respectively. Thus, our data do not support the hypothesis of insufficient SARS-CoV-2-reactive immunity in critical COVID-19. Conversely, it indicates that activation of differentiated memory effector T cells could cause hyperreactivity and immunopathogenesis in critical patients.http://www.sciencedirect.com/science/article/pii/S266637912030118XCOVID-19SARS-CoV-2immunityspikenucleocapsidS/M/N protein-reactive T cells |