Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma
Introduction: Salivary gland tumors are extremely rare neoplasms. Given their pathohistological image, these tumors represent a great diagnostic challenge. The aim of our study was to differentiate these three tumor types by applying the immunohistochemical and morphometric analysis. Material and Me...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Medicinski fakultet i Klinika za stomatologiju, Niš
2017-01-01
|
Series: | Acta Stomatologica Naissi |
Subjects: | |
Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/0352-5252/2017/0352-52521775676Z.pdf |
id |
doaj-e9cce8ad302747c7b5915d5dce36450d |
---|---|
record_format |
Article |
spelling |
doaj-e9cce8ad302747c7b5915d5dce36450d2020-11-24T22:52:01ZengMedicinski fakultet i Klinika za stomatologiju, NišActa Stomatologica Naissi0352-52521820-12022017-01-0133751676169510.5937/asn1775676Z0352-52521775676ZImmunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinomaŽivković Nikola D.0Mihailović Dragan S.1Kostić Miloš S.2Cvetanović Ana S.3Denčić Tijana V.4Jovičić-Milentijević Maja V.5Mijović Žaklina Ž.6Stojanović Simona M.7Spasić Milan S.8Pešić Zoran U.9Trajković Miloš N.10Centar za patologiju i patološku anatomiju, Klinički centar, Niš + Univerzitet u Nišu, Medicinski fakultetCentar za patologiju i patološku anatomiju, Klinički centar, Niš + Univerzitet u Nišu, Medicinski fakultetCentar za patologiju i patološku anatomiju, Klinički centar, Niš + Univerzitet u Nišu, Institut za imunologiju, Medicinski fakultetCentar za patologiju i patološku anatomiju, Klinički centar, Niš + Klinika za onkologiju, Klinički centar, NišCentar za patologiju i patološku anatomiju, Klinički centar, Niš + Univerzitet u Nišu, Medicinski fakultetCentar za patologiju i patološku anatomiju, Klinički centar, Niš + Univerzitet u Nišu, Medicinski fakultetCentar za patologiju i patološku anatomiju, Klinički centar, Niš + Univerzitet u Nišu, Medicinski fakultetUniveristy of Niš, Faculty of Medicine, Niš, SerbiaUniversity of Niš, Faculty of Medicine - Stomatology Clinics, Niš, SerbiaUniverzitet u Nišu, Medicinski fakultet + Odeljenje za maksilofacijalnu hirurgiju, Klinika za stomatologiju, NišUniversity of Niš, Faculty of Medicine - Stomatology Clinics, Niš, SerbiaIntroduction: Salivary gland tumors are extremely rare neoplasms. Given their pathohistological image, these tumors represent a great diagnostic challenge. The aim of our study was to differentiate these three tumor types by applying the immunohistochemical and morphometric analysis. Material and Methods: The entire study was conducted at the Center for Pathology and Pathological Anatomy in Niš. The study included 44 tumors, 20 pleomorphic adenomas, 12 basal cells adenomas and 12 polymorphous low-grade adenocarcinomas. The expression of Ki67, p53 and HER-2 antigens, as proliferation markers, was analyzed. The differential diagnostics also included the analysis of the expression of CEA, EMA, GFAP, p63, vimentin, CK14, α-SMA, S-100 protein and WT1 antigen. The morphometric analysis was done in the program pack 'ImageJ' version 1.43u. The statistical analysis of data was done in the program pack SPSS 15.0. Results: Neoplastic cells in pleomorphic adenoma showed a strong expression of GFAP (20/20), p63 (20/20), WT1 (20/20), vimentin (18/20) and S100 (16/20). Diffuse expression of CK14 (12/12) was present in the group of 12 polymorphous low-grade adenocarcinomas. S-100, vimentin and EMA were absolutely expressed, whereas α-SMA was negative. Basal cell adenoma showed negativity to S-100, CEA, p63 and vimentin. The analysis of the proliferative Ki67 index values pointed to a statistically significant difference in the pleomorphic adenoma group, which was associated with a frequent recurrence of this benign tumor. The analysis of morphometric characteristics showed higher values in the polymorphous low-grade adenocarcinoma group, but statistically significant differences were found only for the Feret diameter and the integrated optical density (p < 0.05). As for the basal cell adenoma group, tumor cells showed statistically higher values for the integrated optical density (p < 0.001). Conclusion: Apart from the basic micromorphological analysis, the differential analysis of salivary gland tumors also requires the immunohistochemical analysis as well as the monitoring of morphometric characteristics of these tumors' nuclei.http://scindeks-clanci.ceon.rs/data/pdf/0352-5252/2017/0352-52521775676Z.pdfpleomorphic adenomapolymorphous low-grade adenomabasal cell adenomaimmunohistochemistrymorphometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Živković Nikola D. Mihailović Dragan S. Kostić Miloš S. Cvetanović Ana S. Denčić Tijana V. Jovičić-Milentijević Maja V. Mijović Žaklina Ž. Stojanović Simona M. Spasić Milan S. Pešić Zoran U. Trajković Miloš N. |
spellingShingle |
Živković Nikola D. Mihailović Dragan S. Kostić Miloš S. Cvetanović Ana S. Denčić Tijana V. Jovičić-Milentijević Maja V. Mijović Žaklina Ž. Stojanović Simona M. Spasić Milan S. Pešić Zoran U. Trajković Miloš N. Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma Acta Stomatologica Naissi pleomorphic adenoma polymorphous low-grade adenoma basal cell adenoma immunohistochemistry morphometry |
author_facet |
Živković Nikola D. Mihailović Dragan S. Kostić Miloš S. Cvetanović Ana S. Denčić Tijana V. Jovičić-Milentijević Maja V. Mijović Žaklina Ž. Stojanović Simona M. Spasić Milan S. Pešić Zoran U. Trajković Miloš N. |
author_sort |
Živković Nikola D. |
title |
Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma |
title_short |
Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma |
title_full |
Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma |
title_fullStr |
Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma |
title_full_unstemmed |
Immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma |
title_sort |
immunohistochemical and karyometric similarities and differences of salivary gland tumors between pleomorphic adenoma, basal cell adenoma and polymorphous low grade adenocarcinoma |
publisher |
Medicinski fakultet i Klinika za stomatologiju, Niš |
series |
Acta Stomatologica Naissi |
issn |
0352-5252 1820-1202 |
publishDate |
2017-01-01 |
description |
Introduction: Salivary gland tumors are extremely rare neoplasms. Given their pathohistological image, these tumors represent a great diagnostic challenge. The aim of our study was to differentiate these three tumor types by applying the immunohistochemical and morphometric analysis. Material and Methods: The entire study was conducted at the Center for Pathology and Pathological Anatomy in Niš. The study included 44 tumors, 20 pleomorphic adenomas, 12 basal cells adenomas and 12 polymorphous low-grade adenocarcinomas. The expression of Ki67, p53 and HER-2 antigens, as proliferation markers, was analyzed. The differential diagnostics also included the analysis of the expression of CEA, EMA, GFAP, p63, vimentin, CK14, α-SMA, S-100 protein and WT1 antigen. The morphometric analysis was done in the program pack 'ImageJ' version 1.43u. The statistical analysis of data was done in the program pack SPSS 15.0. Results: Neoplastic cells in pleomorphic adenoma showed a strong expression of GFAP (20/20), p63 (20/20), WT1 (20/20), vimentin (18/20) and S100 (16/20). Diffuse expression of CK14 (12/12) was present in the group of 12 polymorphous low-grade adenocarcinomas. S-100, vimentin and EMA were absolutely expressed, whereas α-SMA was negative. Basal cell adenoma showed negativity to S-100, CEA, p63 and vimentin. The analysis of the proliferative Ki67 index values pointed to a statistically significant difference in the pleomorphic adenoma group, which was associated with a frequent recurrence of this benign tumor. The analysis of morphometric characteristics showed higher values in the polymorphous low-grade adenocarcinoma group, but statistically significant differences were found only for the Feret diameter and the integrated optical density (p < 0.05). As for the basal cell adenoma group, tumor cells showed statistically higher values for the integrated optical density (p < 0.001). Conclusion: Apart from the basic micromorphological analysis, the differential analysis of salivary gland tumors also requires the immunohistochemical analysis as well as the monitoring of morphometric characteristics of these tumors' nuclei. |
topic |
pleomorphic adenoma polymorphous low-grade adenoma basal cell adenoma immunohistochemistry morphometry |
url |
http://scindeks-clanci.ceon.rs/data/pdf/0352-5252/2017/0352-52521775676Z.pdf |
work_keys_str_mv |
AT zivkovicnikolad immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT mihailovicdragans immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT kosticmiloss immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT cvetanovicanas immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT dencictijanav immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT jovicicmilentijevicmajav immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT mijoviczaklinaz immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT stojanovicsimonam immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT spasicmilans immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT pesiczoranu immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma AT trajkovicmilosn immunohistochemicalandkaryometricsimilaritiesanddifferencesofsalivaryglandtumorsbetweenpleomorphicadenomabasalcelladenomaandpolymorphouslowgradeadenocarcinoma |
_version_ |
1725667565533921280 |