Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane
Background: Mesenchymal stem cells (MSCs) have a capacity for self-renewal and multi-potential differentiations. These cells are considered powerful sources for cell therapy in regenerative medicine and tissue engineering. The cells can be isolated from various tissues; however, harvesting from huma...
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Shiraz University of Medical Sciences
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doaj-e9cfd10e308b40898f47c40019bbe3462020-11-25T02:46:51ZengShiraz University of Medical SciencesInternational Journal of Organ Transplantation Medicine2008-64822008-64902013-07-0143127Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic MembraneT Pirjali0N Azarpira1M Ayatollahi2MH Aghdai3B Geramizadeh4T Talai5Transplant Research Center, Shiraz University of Medical Science, Shiraz, IranTransplant Research Center, Shiraz University of Medical Science, Shiraz, IranTransplant Research Center, Shiraz University of Medical Science, Shiraz, IranTransplant Research Center, Shiraz University of Medical Science, Shiraz, IranTransplant Research Center, Shiraz University of Medical Science, Shiraz, IranShiraz Institute of Stem Cell and Regenerative Medicine, Shiraz University of Medical Science, Shiraz, IranBackground: Mesenchymal stem cells (MSCs) have a capacity for self-renewal and multi-potential differentiations. These cells are considered powerful sources for cell therapy in regenerative medicine and tissue engineering. The cells can be isolated from various tissues; however, harvesting from human umbilical cord and amniotic membrane is easy and accessible source. Objective: To isolate and characterize the MSCs derived from human umbilical cord Wharton’s jelly (WJMSC) and amniotic membrane (AM-MSC) with regard to their morphology, immunophenotype and mesodermal differentiation potential in order to obtain an alternative source of MSC for therapeutic clinical applications. Methods: Fetal membranes and umbilical cords (n=3) were retrieved from healthy full-term women by elective cesarean delivery. Amniotic membrane and umbilical cord were separately minced and cultured in DMEM supplemented with 10% FBS. After reaching 80% of confluency, the umbilical cord WJ-MSC and AM-MSC were characterized by expression of cell surface markers with flowcytometry, stem cell gene expression with adipogenic/osteogenic potential. Results: Both WJ-MSC and AM-MSC were spindle-shaped cells, expressed MSC surface markers in flowcytometry and stem cell transcriptional factors (OCT4 and NANOG). After induction, the cells differentiated into adipogenic and osteogenic lineages. Conclusion: MSC were successfully generated from umbilical cord WJ-MSC and AM-MSC with similar mesenchymal markers and properties.http://www.ijotm.com/ojs/index.php/IJOTM/article/view/138Mesenchymal Stromal cellsAmnionUmbilical cord |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
T Pirjali N Azarpira M Ayatollahi MH Aghdai B Geramizadeh T Talai |
spellingShingle |
T Pirjali N Azarpira M Ayatollahi MH Aghdai B Geramizadeh T Talai Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane International Journal of Organ Transplantation Medicine Mesenchymal Stromal cells Amnion Umbilical cord |
author_facet |
T Pirjali N Azarpira M Ayatollahi MH Aghdai B Geramizadeh T Talai |
author_sort |
T Pirjali |
title |
Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane |
title_short |
Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane |
title_full |
Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane |
title_fullStr |
Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane |
title_full_unstemmed |
Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton�s Jelly and Amniotic Membrane |
title_sort |
isolation and characterization of human mesenchymal stem cells derived from human umbilical cord wharton�s jelly and amniotic membrane |
publisher |
Shiraz University of Medical Sciences |
series |
International Journal of Organ Transplantation Medicine |
issn |
2008-6482 2008-6490 |
publishDate |
2013-07-01 |
description |
Background: Mesenchymal stem cells (MSCs) have a capacity for self-renewal and multi-potential differentiations. These cells are considered powerful sources for cell therapy in regenerative medicine and tissue engineering. The cells can be isolated from various tissues; however, harvesting from human umbilical cord and amniotic membrane is easy and accessible source.
Objective: To isolate and characterize the MSCs derived from human umbilical cord Wharton’s jelly (WJMSC) and amniotic membrane (AM-MSC) with regard to their morphology, immunophenotype and mesodermal differentiation potential in order to obtain an alternative source of MSC for therapeutic clinical applications.
Methods: Fetal membranes and umbilical cords (n=3) were retrieved from healthy full-term women by elective cesarean delivery. Amniotic membrane and umbilical cord were separately minced and cultured in DMEM supplemented with 10% FBS. After reaching 80% of confluency, the umbilical cord WJ-MSC and AM-MSC were characterized by expression of cell surface markers with flowcytometry, stem cell gene expression with adipogenic/osteogenic potential.
Results: Both WJ-MSC and AM-MSC were spindle-shaped cells, expressed MSC surface markers in flowcytometry and stem cell transcriptional factors (OCT4 and NANOG). After induction, the cells differentiated into adipogenic and osteogenic lineages.
Conclusion: MSC were successfully generated from umbilical cord WJ-MSC and AM-MSC with similar mesenchymal markers and properties. |
topic |
Mesenchymal Stromal cells Amnion Umbilical cord |
url |
http://www.ijotm.com/ojs/index.php/IJOTM/article/view/138 |
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