The Effect of the Anticipated Nuclear Localization Sequence of ‘<i>Candidatus</i> Phytoplasma mali’ SAP11-like Protein on Localization of the Protein and Destabilization of TCP Transcription Factor

• Main Authors: Alisa Strohmayer, Timothy Schwarz, Mario Braun, Gabi Krczal, Kajohn Boonrod
• Format: Article
• Language: English
• Published: MDPI AG 2021-08-01
• Series: Microorganisms
• Subjects: phytoplasmas; ‘<i>Candidatus</i> Phytoplasma mali’; SAP11; plant-pathogen interaction; TCP transcription factors
• Online Access: https://www.mdpi.com/2076-2607/9/8/1756

SAP11 is an effector protein that has been identified in various phytoplasma species. It localizes in the plant nucleus and can bind and destabilize TEOSINE BRANCHES/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors. Although SAP11 of different phytoplasma species share similar activities, their protein sequences differ greatly. Here, we demonstrate that the SAP11-like protein of ‘<i>Candidatus</i> Phytoplasma mali’ (‘<i>Ca</i>. P. mali’) strain PM19 localizes into the plant nucleus without requiring the anticipated nuclear localization sequence (NLS). We show that the protein induces crinkled leaves and siliques, and witches’ broom symptoms, in transgenic <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) plants and binds to six members of class I and all members of class II TCP transcription factors of <i>A. thaliana</i> in yeast two-hybrid assays. We also identified a 17 amino acid stretch previously predicted to be a nuclear localization sequence that is important for the binding of some of the TCPs, which results in a crinkled leaf and silique phenotype in transgenic <i>A. thaliana</i>. Moreover, we provide evidence that the SAP11-like protein has a destabilizing effect on some TCPs in vivo.