Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila.
Intragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3'UTR...
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2015-05-01
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doaj-e9ec18847de64f82b8e79d61b1a284022020-11-24T21:19:12ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-05-01115e100526410.1371/journal.pgen.1005264Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila.Yeliz Yuva-AydemirXia-Lian XuOzkan AydemirEduardo GasconSerkan SayinWenke ZhouYang HongFen-Biao GaoIntragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3'UTR of jigr1, respectively, and co-expressed with some jigr1 isoforms. miR-92a and miR-92b are highly expressed in neuroblasts of larval brain where Jigr1 expression is low. Genetic deletion of both miR-92a and miR-92b demonstrates an essential cell-autonomous role for these miRNAs in maintaining neuroblast self-renewal through inhibiting premature differentiation. We also show that miR-92a and miR-92b directly target jigr1 in vivo and that some phenotypes due to the absence of these miRNAs are partially rescued by reducing the level of jigr1. These results reveal a novel function of the miR-92 family in Drosophila neuroblasts and provide another example that local negative feedback regulation of host genes by intragenic miRNAs is essential for animal development.http://europepmc.org/articles/PMC4441384?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yeliz Yuva-Aydemir Xia-Lian Xu Ozkan Aydemir Eduardo Gascon Serkan Sayin Wenke Zhou Yang Hong Fen-Biao Gao |
spellingShingle |
Yeliz Yuva-Aydemir Xia-Lian Xu Ozkan Aydemir Eduardo Gascon Serkan Sayin Wenke Zhou Yang Hong Fen-Biao Gao Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. PLoS Genetics |
author_facet |
Yeliz Yuva-Aydemir Xia-Lian Xu Ozkan Aydemir Eduardo Gascon Serkan Sayin Wenke Zhou Yang Hong Fen-Biao Gao |
author_sort |
Yeliz Yuva-Aydemir |
title |
Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. |
title_short |
Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. |
title_full |
Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. |
title_fullStr |
Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. |
title_full_unstemmed |
Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. |
title_sort |
downregulation of the host gene jigr1 by mir-92 is essential for neuroblast self-renewal in drosophila. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2015-05-01 |
description |
Intragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3'UTR of jigr1, respectively, and co-expressed with some jigr1 isoforms. miR-92a and miR-92b are highly expressed in neuroblasts of larval brain where Jigr1 expression is low. Genetic deletion of both miR-92a and miR-92b demonstrates an essential cell-autonomous role for these miRNAs in maintaining neuroblast self-renewal through inhibiting premature differentiation. We also show that miR-92a and miR-92b directly target jigr1 in vivo and that some phenotypes due to the absence of these miRNAs are partially rescued by reducing the level of jigr1. These results reveal a novel function of the miR-92 family in Drosophila neuroblasts and provide another example that local negative feedback regulation of host genes by intragenic miRNAs is essential for animal development. |
url |
http://europepmc.org/articles/PMC4441384?pdf=render |
work_keys_str_mv |
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