Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells
This study aimed to investigate the anti-inflammatory properties of sodium phenylbutyrate (SPB) against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) lipoteichoic acid (LTA)-stimulated bovine mammary alveolar (MAC-T) cells. Quantitative PCR was performed to examine the effe...
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2018-11-01
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doaj-ea025057d5814b3a82bf678a756473d12020-11-24T21:21:07ZengMDPI AGMolecules1420-30492018-11-012312305610.3390/molecules23123056molecules23123056Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T CellsXin Wang0Mengmeng Zhang1Ning Jiang2Aizhong Zhang3College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing 163319, ChinaCollege of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing 163319, ChinaCollege of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing 163319, ChinaCollege of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing 163319, ChinaThis study aimed to investigate the anti-inflammatory properties of sodium phenylbutyrate (SPB) against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) lipoteichoic acid (LTA)-stimulated bovine mammary alveolar (MAC-T) cells. Quantitative PCR was performed to examine the effect of SPB on inflammatory cytokines and host defense peptide (HDP) gene expression. Western blot wanalysis was used to detect the effect of SPB on the TLR2/NF-κB/NLRP3 signaling pathway. The results showed that SPB significantly suppressed the expression of TNF-α, IL-1β, IL-6; meanwhile, the markedly decreased expression of LTA-stimulated TLR2, NLRP3, ASC, caspase-1, and IL-1β, and the inhibited IkBα and p65 phosphorylation were also observed. However, increased TAP and Bac5 expression in LTA-stimulated MAC-T cells was further detected. In summary, these results suggest that SPB ameliorates the inflammatory response induced by <i>S. aureus</i> LTA via suppressing the TLR2/NF-κB/NLRP3 signaling pathway, which indicates that SPB may be a potential agent for the treatment of bovine mastitis.https://www.mdpi.com/1420-3049/23/12/3056sodium phenylbutyratelipoteichoic acidinflammationTLR2NF-κBNLRP3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Wang Mengmeng Zhang Ning Jiang Aizhong Zhang |
spellingShingle |
Xin Wang Mengmeng Zhang Ning Jiang Aizhong Zhang Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells Molecules sodium phenylbutyrate lipoteichoic acid inflammation TLR2 NF-κB NLRP3 |
author_facet |
Xin Wang Mengmeng Zhang Ning Jiang Aizhong Zhang |
author_sort |
Xin Wang |
title |
Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells |
title_short |
Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells |
title_full |
Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells |
title_fullStr |
Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells |
title_full_unstemmed |
Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells |
title_sort |
sodium phenylbutyrate ameliorates inflammatory response induced by <i>staphylococcus aureus</i> lipoteichoic acid via suppressing tlr2/nf-κb/nlrp3 pathways in mac-t cells |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-11-01 |
description |
This study aimed to investigate the anti-inflammatory properties of sodium phenylbutyrate (SPB) against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) lipoteichoic acid (LTA)-stimulated bovine mammary alveolar (MAC-T) cells. Quantitative PCR was performed to examine the effect of SPB on inflammatory cytokines and host defense peptide (HDP) gene expression. Western blot wanalysis was used to detect the effect of SPB on the TLR2/NF-κB/NLRP3 signaling pathway. The results showed that SPB significantly suppressed the expression of TNF-α, IL-1β, IL-6; meanwhile, the markedly decreased expression of LTA-stimulated TLR2, NLRP3, ASC, caspase-1, and IL-1β, and the inhibited IkBα and p65 phosphorylation were also observed. However, increased TAP and Bac5 expression in LTA-stimulated MAC-T cells was further detected. In summary, these results suggest that SPB ameliorates the inflammatory response induced by <i>S. aureus</i> LTA via suppressing the TLR2/NF-κB/NLRP3 signaling pathway, which indicates that SPB may be a potential agent for the treatment of bovine mastitis. |
topic |
sodium phenylbutyrate lipoteichoic acid inflammation TLR2 NF-κB NLRP3 |
url |
https://www.mdpi.com/1420-3049/23/12/3056 |
work_keys_str_mv |
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