Sodium Phenylbutyrate Ameliorates Inflammatory Response Induced by <i>Staphylococcus aureus</i> Lipoteichoic Acid via Suppressing TLR2/NF-κB/NLRP3 Pathways in MAC-T Cells

• Main Authors: Xin Wang, Mengmeng Zhang, Ning Jiang, Aizhong Zhang
• Format: Article
• Language: English
• Published: MDPI AG 2018-11-01
• Series: Molecules
• Subjects: sodium phenylbutyrate; lipoteichoic acid; inflammation; TLR2; NF-κB; NLRP3
• Online Access: https://www.mdpi.com/1420-3049/23/12/3056

This study aimed to investigate the anti-inflammatory properties of sodium phenylbutyrate (SPB) against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) lipoteichoic acid (LTA)-stimulated bovine mammary alveolar (MAC-T) cells. Quantitative PCR was performed to examine the effect of SPB on inflammatory cytokines and host defense peptide (HDP) gene expression. Western blot wanalysis was used to detect the effect of SPB on the TLR2/NF-&#954;B/NLRP3 signaling pathway. The results showed that SPB significantly suppressed the expression of TNF-&#945;, IL-1&#946;, IL-6; meanwhile, the markedly decreased expression of LTA-stimulated TLR2, NLRP3, ASC, caspase-1, and IL-1&#946;, and the inhibited IkB&#945; and p65 phosphorylation were also observed. However, increased TAP and Bac5 expression in LTA-stimulated MAC-T cells was further detected. In summary, these results suggest that SPB ameliorates the inflammatory response induced by <i>S. aureus</i> LTA via suppressing the TLR2/NF-&#954;B/NLRP3 signaling pathway, which indicates that SPB may be a potential agent for the treatment of bovine mastitis.