Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid
Asian honey bee (<i>Apis cerana</i>) is the most important Chinese indigenous species, while its toxicological characteristic against neonicotinoids is poorly known. Here, we combined physiological experiments with a genome-wide transcriptome analysis to understand the molecular basis of...
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doaj-ea0ad979ded8450992186058522feb552020-11-25T03:08:41ZengMDPI AGInsects2075-44502020-11-011175375310.3390/insects11110753Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid ImidaclopridJing Gao0San-Sheng Jin1Yan He2Jin-Hong Luo3Chun-Qin Xu4Yan-Yan Wu5Chun-Shen Hou6Qiang Wang7Qing-Yun Diao8Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaKey Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaNational Maize Improvement Center of China, Beijing Key Laboratory of crop genetic Improvement, China Agricultural University, Beijing 100083, ChinaNational Maize Improvement Center of China, Beijing Key Laboratory of crop genetic Improvement, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaKey Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaKey Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaKey Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaKey Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaAsian honey bee (<i>Apis cerana</i>) is the most important Chinese indigenous species, while its toxicological characteristic against neonicotinoids is poorly known. Here, we combined physiological experiments with a genome-wide transcriptome analysis to understand the molecular basis of genetic variation that responds to sublethal imidacloprid at different exposure durations in <i>A. cerana.</i> We found that LC<sub>5</sub> dose of imidacloprid had a negative impact on climbing ability and sucrose responsiveness in <i>A. cerana</i>. When bees were fed with LC<sub>5</sub> dose of imidacloprid, the enzyme activities of P450 and CarE were decreased, while the GSTs activity was not influenced by the pesticide exposure. The dynamic transcriptomic profiles of <i>A. cerana</i> workers exposed to LC<sub>5</sub> dose of imidacloprid for 1 h, 8 h, and 16 h were obtained by high-throughput RNA-sequencing. We performed the expression patterns of differentially expressed genes (DEGs) through trend analysis, and conducted the gene ontology analysis and KEGG pathway enrichment analysis with DEGs in up- and down-regulated pattern profiles. We observed that more genes involved in metabolism, catalytic activity, and structural molecule activity are down-regulated; while more up-regulated genes were enriched in terms associated with response to stimulus, transporter activity, and signal transducer activity. Additionally, genes related to the phenylalanine metabolism pathway, FoxO signaling pathway, and mTOR signaling pathway as indicated in the KEGG analysis were significantly up-related in the exposed bees. Our findings provide a comprehensive understanding of Asian honey bee in response to neonicotinoids sublethal toxicity, and could be used to further investigate the complex molecular mechanisms in Asian honey bee under pesticide stress.https://www.mdpi.com/2075-4450/11/11/753<i>Apis cerana</i>ImidaclopridDetoxificationtranscriptome analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Gao San-Sheng Jin Yan He Jin-Hong Luo Chun-Qin Xu Yan-Yan Wu Chun-Shen Hou Qiang Wang Qing-Yun Diao |
spellingShingle |
Jing Gao San-Sheng Jin Yan He Jin-Hong Luo Chun-Qin Xu Yan-Yan Wu Chun-Shen Hou Qiang Wang Qing-Yun Diao Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid Insects <i>Apis cerana</i> Imidacloprid Detoxification transcriptome analysis |
author_facet |
Jing Gao San-Sheng Jin Yan He Jin-Hong Luo Chun-Qin Xu Yan-Yan Wu Chun-Shen Hou Qiang Wang Qing-Yun Diao |
author_sort |
Jing Gao |
title |
Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid |
title_short |
Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid |
title_full |
Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid |
title_fullStr |
Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid |
title_full_unstemmed |
Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (<i>Apis cerana cerana</i>) in Response to Sublethal Neonicotinoid Imidacloprid |
title_sort |
physiological analysis and transcriptome analysis of asian honey bee (<i>apis cerana cerana</i>) in response to sublethal neonicotinoid imidacloprid |
publisher |
MDPI AG |
series |
Insects |
issn |
2075-4450 |
publishDate |
2020-11-01 |
description |
Asian honey bee (<i>Apis cerana</i>) is the most important Chinese indigenous species, while its toxicological characteristic against neonicotinoids is poorly known. Here, we combined physiological experiments with a genome-wide transcriptome analysis to understand the molecular basis of genetic variation that responds to sublethal imidacloprid at different exposure durations in <i>A. cerana.</i> We found that LC<sub>5</sub> dose of imidacloprid had a negative impact on climbing ability and sucrose responsiveness in <i>A. cerana</i>. When bees were fed with LC<sub>5</sub> dose of imidacloprid, the enzyme activities of P450 and CarE were decreased, while the GSTs activity was not influenced by the pesticide exposure. The dynamic transcriptomic profiles of <i>A. cerana</i> workers exposed to LC<sub>5</sub> dose of imidacloprid for 1 h, 8 h, and 16 h were obtained by high-throughput RNA-sequencing. We performed the expression patterns of differentially expressed genes (DEGs) through trend analysis, and conducted the gene ontology analysis and KEGG pathway enrichment analysis with DEGs in up- and down-regulated pattern profiles. We observed that more genes involved in metabolism, catalytic activity, and structural molecule activity are down-regulated; while more up-regulated genes were enriched in terms associated with response to stimulus, transporter activity, and signal transducer activity. Additionally, genes related to the phenylalanine metabolism pathway, FoxO signaling pathway, and mTOR signaling pathway as indicated in the KEGG analysis were significantly up-related in the exposed bees. Our findings provide a comprehensive understanding of Asian honey bee in response to neonicotinoids sublethal toxicity, and could be used to further investigate the complex molecular mechanisms in Asian honey bee under pesticide stress. |
topic |
<i>Apis cerana</i> Imidacloprid Detoxification transcriptome analysis |
url |
https://www.mdpi.com/2075-4450/11/11/753 |
work_keys_str_mv |
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