Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification
Upscaling of biomass pretreatment from laboratory scale to a bench-scale reactor is one of the important steps in the application of the pretreatment for pilot or commercial scale. This study reports the optimization of pretreatment conditions, namely reaction temperature and time, by one factor at...
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Diponegoro University
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doaj-ea15d4ccac6e4ae4b164160ab57515312021-02-18T23:19:45ZengDiponegoro UniversityInternational Journal of Renewable Energy Development2252-49402021-05-0110215716910.14710/ijred.2021.3234317518Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic SaccharificationFahriya Puspita Sari0Faizatul Falah1Sita Heris Anita2Kharisma Panji Ramadhan3Raden Permana Budi Laksana4Widya Fatriasari5Euis Hermiati6Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Research Center for Biomaterials LIPI, Jl Raya Bogor KM 46 Cibinong 16911Upscaling of biomass pretreatment from laboratory scale to a bench-scale reactor is one of the important steps in the application of the pretreatment for pilot or commercial scale. This study reports the optimization of pretreatment conditions, namely reaction temperature and time, by one factor at a time (OFAT) method for the enhancement of enzymatic saccharification of oil palm empty fruit bunch (OPEFB). OPEFB was pretreated using high temperature-pressure steam reactor with different reaction temperatures (160, 170, 180, 190, 200 °C) and times (10, 20, 30, 40, 50 min). The effectiveness of the pretreatment was determined based on chemical compositions of raw OPEFB and OPEFB pulp and sugar production from enzymatic saccharification of the OPEFB pulp. Solubilized components from OPEFB, such as glucose, xylose, formic acid, acetic acid, 5-hydroxymethyl furfural (HMF), and furfural in the hydrolysate that generated during steam pretreatment were also determined. Pretreatment at 180°C for 20 min provides the highest sugar yields (97.30% of glucose yield per initial cellulose and 88.86% of xylose yield per initial hemicellulose). At the optimum condition, 34.9% of lignin and 30.75% of hemicellulose are successfully removed from the OPEFB and resulted in 3.43 delignification selectivity. The relationship between severity factor and by-products generated and the sugars obtained after enzymatic saccharification are discussed. The pulp of OPEFB at the optimum condition was also characterized for its morphological characteristic by scanning electron microscopy (SEM) and crystallinity by X-ray diffractometry (XRD). These pulp characteristics are then compared with those of the raw OPEFB. The steam pretreatment causes some fiber disruptions with more defined and opened structures and increases the crystallinity index (CrI) by 2.9% compared to the raw OPEFB.https://ejournal.undip.ac.id/index.php/ijred/article/view/32343steam pretreatmentbench scale reactorenzymatic saccharificationopefb |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fahriya Puspita Sari Faizatul Falah Sita Heris Anita Kharisma Panji Ramadhan Raden Permana Budi Laksana Widya Fatriasari Euis Hermiati |
spellingShingle |
Fahriya Puspita Sari Faizatul Falah Sita Heris Anita Kharisma Panji Ramadhan Raden Permana Budi Laksana Widya Fatriasari Euis Hermiati Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification International Journal of Renewable Energy Development steam pretreatment bench scale reactor enzymatic saccharification opefb |
author_facet |
Fahriya Puspita Sari Faizatul Falah Sita Heris Anita Kharisma Panji Ramadhan Raden Permana Budi Laksana Widya Fatriasari Euis Hermiati |
author_sort |
Fahriya Puspita Sari |
title |
Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification |
title_short |
Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification |
title_full |
Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification |
title_fullStr |
Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification |
title_full_unstemmed |
Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification |
title_sort |
pretreatment of oil palm empty fruit bunch (opefb) at bench-scale high temperature-pressure steam reactor for enhancement of enzymatic saccharification |
publisher |
Diponegoro University |
series |
International Journal of Renewable Energy Development |
issn |
2252-4940 |
publishDate |
2021-05-01 |
description |
Upscaling of biomass pretreatment from laboratory scale to a bench-scale reactor is one of the important steps in the application of the pretreatment for pilot or commercial scale. This study reports the optimization of pretreatment conditions, namely reaction temperature and time, by one factor at a time (OFAT) method for the enhancement of enzymatic saccharification of oil palm empty fruit bunch (OPEFB). OPEFB was pretreated using high temperature-pressure steam reactor with different reaction temperatures (160, 170, 180, 190, 200 °C) and times (10, 20, 30, 40, 50 min). The effectiveness of the pretreatment was determined based on chemical compositions of raw OPEFB and OPEFB pulp and sugar production from enzymatic saccharification of the OPEFB pulp. Solubilized components from OPEFB, such as glucose, xylose, formic acid, acetic acid, 5-hydroxymethyl furfural (HMF), and furfural in the hydrolysate that generated during steam pretreatment were also determined. Pretreatment at 180°C for 20 min provides the highest sugar yields (97.30% of glucose yield per initial cellulose and 88.86% of xylose yield per initial hemicellulose). At the optimum condition, 34.9% of lignin and 30.75% of hemicellulose are successfully removed from the OPEFB and resulted in 3.43 delignification selectivity. The relationship between severity factor and by-products generated and the sugars obtained after enzymatic saccharification are discussed. The pulp of OPEFB at the optimum condition was also characterized for its morphological characteristic by scanning electron microscopy (SEM) and crystallinity by X-ray diffractometry (XRD). These pulp characteristics are then compared with those of the raw OPEFB. The steam pretreatment causes some fiber disruptions with more defined and opened structures and increases the crystallinity index (CrI) by 2.9% compared to the raw OPEFB. |
topic |
steam pretreatment bench scale reactor enzymatic saccharification opefb |
url |
https://ejournal.undip.ac.id/index.php/ijred/article/view/32343 |
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